Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...

In protein NMR the assignment of nuclear spin resonances is a prerequisite for all subsequent applications, such as studies of ligand binding, protein-DNA interactions, and dynamics. Resonance assignment is a time consuming step even when the 3D x-ray structure of the protein is available. A new strategy is presented to solve the "inverse" assignment problem, which is the determination of the N...

The paper proposes a new formulation of the output feedback pole assignment problem. In this formulation, a unified approach is presented for solving the pole assignment problem with various additional objectives. These objectives include optimizing a variety of performance indices, and imposing constraints on the output feedback matrix structure, e.g. decentralized structure. Conditions for th...

Classifier fusion techniques are gaining more popularity for their capability of improving the accuracy achieved by individual classifiers. A common approach is to combine the classifiers’ outcome using simple methods, such as majority voting. In this paper, we build a meta-classifier by fusing some already well-known classifiers for protein structure prediction. Each individual classifier outp...

Sequential assignments are reported for backbone 15N and 1H of nearly all residues of staphylococcal nuclease (Nase) complexed with thymidine 3',5'-diphosphate and Ca2+. Because of the relatively large size of the Nase ternary complex, Mr 18K, the crucial element of our assignment strategy was the use of isotope-edited two-dimensional NMR spectra, particularly 15N-edited nuclear Overhauser enha...

A sensor network consists of a set of battery-powered nodes, which collaborate to perform sensing tasks in a given environment. Globally unique ID allocation is usually not applicable in a sensor network due to the massive production of cheap sensor nodes, the limited bandwidth, and the size of the payload. However, locally unique IDs are still necessary for nodes to implement communications to...

The structure in solution of cardiotoxin III, a membrane toxin purified from the venom of the Taiwan cobra, Naja naja atra, is reported. Sequence-specific assignment of 1H-NMR lines was completed and the NMR data show the presence of a triple and a double-stranded antiparallel beta-sheet. Many NOE cross peaks identified in NOESY spectra were applied as distance constraints based on a hybrid dis...