background : the protozoan parasites cryptosporidium spp. and giardia are known to occur widely in both raw and drinking waters. they are two of the causative agents of waterborne out-breaks of gastroenteritis throughout the world. in the present study, a pcr assay and fa were developed for detection of cryptosporidium oocysts and giardia cyst in environmental samples. methods : we have detecte...

conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...

background: babesia is blood-tissue parasite, which are transmitted by hard ticks from ixodidae family. the parasite is the cause of babesiosis among ruminants. babesia is as one of main fatal factors among livestock in endemic regions such as iran. the aim of this study was to investigate babesia spp infection using microscopic and molecular methods among small ruminants in mazandaran and gole...

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

BACKGROUND & OBJECTIVE Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS The PCR base...