Background Persistent human bocavirus 1 (HBoV1) infection is a common finding in patients suffering from chronic tonsillar disease. However, the associations between HBoV1 and specific immune reactions are not completely known. We aimed to compare vivo expression of T-cell cytokines, transcription factors, type I/III interferons tonsils HBoV1-positive -negative tonsillectomy patients. Methods T...

A simple method to assess the degree of degradation present in a total RNA preparation from cells or tissues is based on the increasing probability of RNA cleavage with increasing length of an RNA molecule. Under ideal conditions, reverse transcription of a particular mRNA species with oligo-dT as the primer generates a population of cDNAs, terminating at the 5' end of the mRNA if all template ...

the invention of polymerase chain reaction (pcr) has been a milestone in the history of biological and medical sciences. the applications of pcr have not only completely revolutionised the research in the field of molecular genetics as well as animal and plant biotechnology, but the technique has also proved its relevance and ingenious utility in other fields of forensic sciences, molecular sys...