A thermally triggered liposome-based mineralization system is described that is metastable at ambient temperature but rapidly forms calcium phosphate mineral upon warming to physiologic temperature. Mixing of a calcium-loaded lipid vesicle suspension with aqueous inorganic phosphate resulted in a stable liposome suspension whose bulk ionic concentration was highly supersaturated with respect to...

Cationic liposome-nucleic acid complexes, which were originally developed for use as non-viral gene delivery vectors, may now have an equally important application as immunotherapeutic drugs. Recent studies have highlighted the ability of cationic liposomes to potently activate the innate immune system when used to deliver certain Toll-like receptor (TLR) agonists. The immune-enhancing properti...

The solubilization dynamics of dimyristoylphosphatidylcholine (DMPC) liposomes, as induced by sodium dodecyl sulfate (SDS), were investigated; this investigation was motivated by several types of atypical behavior that were observed in the solubilization in this system. The liposomes and surfactants were mixed in a microchip, and the solubilization reaction of each liposome was observed using ...

Background/introduction A major challenge in HIFU mediated Image Guided Drug Delivery (IGDD) is developing accurate means to implement real-time drug delivery control, and to optimize intratumoral drug distribution. To facilitate clinical translation, objectives of this study were to: 1) develop echogenic E-LTSL, a low temperature sensitive liposome co-loaded with an US contrast agent (Perfluor...

The authors have examined the fate of intravitreally injected liposomes in the aphakic, vitrectomized eye of the rabbit. Liposomes labelled with 125[I]-p-hydroxybenzimidylphosphatidylethanolamine were eliminated rapidly from the intraocular fluid. Nonetheless, a significant fraction of these liposomes were found to bind to various ocular tissues including the retina, iris, sclera, and cornea. U...

Three basic amino acid–based cationic lipids bearing a fluorescent naphthalimide moiety and reducible disulfide linkage are synthesized applied as non-viral gene vehicles. Their DNA interactions investigated by agarose-gel retardant ethidium bromide replacement assays. The sizes zeta potentials of the liposome/DNA complexes measured dynamic light scattering. cytotoxicities examined using HeLa 7...

BACKGROUND The authors evaluated the efficacy of liposome bupivacaine in a femoral nerve block (FNB) after total knee arthroplasty. METHODS Part 1: subjects received FNB with 20 ml liposome bupivacaine (67, 133, or 266 mg) or placebo. Part 2: subjects were randomized to FNB with liposome bupivacaine 266 mg or placebo. The primary outcome measure was area under the curve of the numeric rating ...