A repetitive sequence of quiescent fluid layers of differing viscosities through which small spherical Brownian particles move is analyzed in order to illustrate in a simple context how the theory of macrotransport processes, a generalization of Taylor dispersion theory, may be employed to rigorously analyze spatially periodic micropatterned chromatographic separation devices for circumstances ...

Increased throughput in the techniques used to engineer new metabolic pathways in unicellular organisms demands similarly high throughput tools for measuring the effects of these pathways on phenotype. For example, the metabolic engineer is often faced with the challenge of selecting the one genomic perturbation that produces a desired result out of tens of thousands of possibilities [1]. We pr...

First lab-on-chip devices based on active transport by biomolecular motors have been demonstrated for basic detection and sorting applications. However, to fully employ the advantages of such hybrid nanotechnology, versatile spatial and temporal control mechanisms are required. Using a thermo-responsive polymer, we demonstrate the selective starting and stopping of modified microtubules gliding...

We introduce a statistical computing framework to address two important issues in spike sorting: flexible spike shape modeling and realtime spike clustering. In this framework, spikes are detected based on a nonparametric shape distribution; detected spikes are further grouped by an incremental clustering algorithm involving the second-order statistics–covariance matrix. We performed experiment...

New methods toward manipulation and study of membrane species within a lipid bilayer platform are desired because lipid bilayers are able to maintain native conformation and function of intrinsic membrane species. This report demonstrates design and use of a bilayer patterned device for separation, sorting, and categorization of membrane biomolecules based on their affinity for different co-exi...

An impedance spectroscopy-based cell/particle position detection method in microfluidic systems is presented. A single pair of non-parallel surface microelectrodes was utilized to detect the transverse positions of particles/cells flowing in a microchannel without the need for a multi-electrode multi-channel impedance detection. This method can be a simple solution for high-throughput and low-c...

Deterministic lateral displacement (DLD) devices separate micrometer-scale particles in solution based on their size using a laminar microfluidic flow in an array of obstacles. We investigate array geometries with rational row-shift fractions in DLD devices by use of a simple model including both advection and diffusion. Our model predicts multidirectional sorting modes that could be experiment...

abstract- the high production of orange fruit in iran calls for quality sorting of this product as a requirement for entering global markets. this study was devoted to the development of an automatic fruit sorter based on size. the hardware consisted of two units. an image acquisition apparatus equipped with a camera, a robotic arm and controller circuits. the second unit consisted of a robotic...

Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min(-1)) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. The approach is generalizable to other microfluidic ...