The effect of various steroidal compounds on the binding of the androphilic protein to dihydrotestosterone in the cytosol of tissues of human benign prostatic hypertrophy was examined. Androgens, as well as estrogens and gestagens showed an inhibitory effect on the binding. Non-steroidal anti-androgens were revealed to be weak inhibitors on the binding. Two androphilic proteins were observed in...

Rat liver cytosol contains two protein fractions capable of serving as benzo(a)pyrene (BP) carrier in in vitro oxidation of BP (O. Hanson-Painton, M.J. Griffin, and J. Tang, Biochem. Biophys. Res. Commun., 101: 1364-1371, 1981). The role of the smaller carrier fraction in in vitro BP transport and oxidation was studied. Using Sephadex G-100 chromatography, [14C]BP bound to the carrier was shown...

Rat liver nuclei were isolated and sonicated for extraction in order to study the capping of RNA. The guanosine 7-methyltransferase was purified from the extract by hydroxylapatite column chromatography with stepwise addition of phosphate buffer. It was assayed by using as methyl acceptor synthetic G(5')ppp(5')G and S-adenosylmethionine as donor. The enzyme appeared in a sharp peak at 160 mM. T...

In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 Sephadex G-200 anion exchange chromatography. The structural, physicochemical antioxidant properties of the main (OAP-1A) were evaluated. contained 94.3% carbohydrate, 3.56% moisture 2.14% ash result gel permeation chromatography (GPC) showed average molecular weight 593 kD...

Rat liver cytosol contains two protein fractions capable of serving as benzo(a)pyrene (BP) carrier in in vitro oxidation of BP (0. Hanson-Painton, M. J. Griffin, and J. Tang, Biochem. Biophys. Res. Commun., 707: 1364-1371,1981). The role of the smaller carrier fraction in in vitro BP transport and oxidation was studied. Using Sephadex G-100 chromatography, [14C]BP bound to the carrier was shown...

A 60to 125-fold purification of nuclear extracts from rat liver triiodothyronine receptor was achieved by dialysis of 0.4 M NaCl nuclear extracts followed by chromatography on DEAE-Sephadex. Extract was labeled either by injecting tracer [““Iltriiodothyronine into the animal or by direct addition of the tracer in. vitro prior to chromatography. Elution of the column with a linear NaCl gradient ...

Endophytes have continued to gain fame due their ability produce an array of secondary metabolites within the host system with huge untapped pharmacological potentials. This study was carried out further identify and isolate novel therapeutic compounds from Fusarium equiseti, Endophytic fungus isolated leaves Ocimum gratissimum. fungal isolation, fermentation, extraction were using standard lab...

Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH...

Gangliosides were isolated from whole spinal cords and cord myelin of human, cat, and rabbit by a revised methodology. The method included the sequential application of DEAE-Sephadex column chromatography, base treatment, Sephadex G-50 column chromatography, and finally Iatrobeads column chromatography. The human whole spinal cord was found to contain about one-tenth of the ganglioside concentr...

The first and second leaf sheaths of Zea mays L. cv Golden Jubilee were extracted and the extract centrifuged at 100,000g to yield a supernatant or cytosol fraction. Binding of [(3)H]gibberellin A(1) (GA(1)) to a soluble macromolecular component present in the cytosol was demonstrated at 4 degrees C by Sephadex G-200 chromatography. The binding component was of high molecular weight (HMW) and g...