The historical method for the detection of botulinum neurotoxin (BoNT) is represented by the mouse bioassay (MBA) measuring the animal survival rate. Since the endpoint of the MBA is the death of the mice due to paralysis of the respiratory muscle, an ex vivo animal replacement method, called mouse phrenic nerve (MPN) assay, employs the isolated N. phrenicus-hemidiaphragm tissue. Here, BoNT cau...

Plant Growth Promoting Rhizobacteria Pseudomonas and Bacillus are well known biofertilizer due to their ability for solubilizing inorganic phosphate producing phytohormones in soil. However, potential decreased diseases incidence caused by Pratylenchus coffeae has been documented. The objective of this laboratory experiment was formulate solid contained diminuta subtilis. experiments consisted ...

Vegetables are an essential dietary source of nutrients and fiber for the health wellbeing human beings. The demand vegetables has increased substantially in recent years. However, research linked vegetable consumption with various pathogenic bacteria that public concern. present study was designed to isolate identify bacteriological composition fluted pumpkin, bitter leaf, scent leaf commonly ...

Somatic activating GNAS mutations cause McCune-Albright syndrome (MAS). Owing to low mutation abundance, mutant-specific enrichment procedures, such as the peptide nucleic acid (PNA) method, are required to detect mutations in peripheral blood. Next generation sequencing (NGS) can analyze millions of PCR amplicons independently, thus it is expected to detect low-abundance GNAS mutations quantit...

Aim of the study was to screen antimicrobial activity pyrimidine derivative 3-[2-(1-naphthyl)-2-oxoethyl]-6-bromoquinazoline-4(3H)-oh with laboratory cipher VMA–13–06 in relation pathogenic and opportunistic flora. Material methods. Antimicrobial established vitro against strains Staphylococcus aureus, Klebsiella pneumonia, Streptococcus pyogenes, Escherichia coli, Acinetobacter baumannii Citro...