Purpose of the study. Was to analyze levels biogenic amines (serotonin and its metabolite 5-HIAA, dopamine, norepinephrine histamine) in lung tissues patients with cancer previous COVID-19 infection. Patients methods. The study was carried out on samples intact tissues, tumor peritumoral obtained during open biopsy while performing radical surgery from morphologically verified non-small cell (N...

Defining a behavior that requires the function of specific neurons in the free-living nematode Caenorhabditis elegans can allow one to screen for mutations that disrupt the specification or function of those neurons. We identified serotonin-immunoreactive neurons required for tail curling or "turning" behavior exhibited by C. elegans males during mating. Males mutant in three different genes th...

The study validated the application of infrared thermography in a serotonin-induced itch model in rats since the only available method in animal models of itch is the count of scratching bouts. Twenty four adult Sprague-Dawley male rats were used in 3 experiments: 1) local vasomotor response induced by intradermal serotonin (10 l) was evaluated against isotonic saline and Methysergide (10 l); 2...

We describe an isocratic liquid-chromatographic method with amperometric detection for determination of serotonin by rapid sample preparation. Platelet-poor plasma and platelets were injected after a single deproteinization step with perchloric acid. Addition of sodium borohydride to whole blood avoids oxidation of serotonin during the deproteinization step without any chromatographic interfere...

Growing evidence shows that the neurotransmitter serotonin (5-HT) modulates the fine-tuning of neuron development and the establishment of wiring patterns in the brain. However, whether serotonin is involved in the maintenance of neuronal circuitry in the adult brain remains elusive. Here, we use a Tph2fl°x conditional knockout (cKO) mouse line to assess the impact of serotonin depletion during...

Prenatal stress in rats results in structural, physiological and behavioral alterations that persist in adulthood. Serotonin (5-HT) is an important neurotransmitter known to be involved in these prenatal stress-induced behavioral alterations. The aim of the study was to investigate the effects of interrupted synthesis of 5-HT and immobilization stress during different gestational period on brai...

The role of serotonin in memory consolidation was investigated by comparing the serotonin content in the brain of young and old animals which differed in the duration of consolidation process and by comparing the serotonin content in the brain with the efficiency of retrieval of memory traces in animals of the same age. Old rats had the serotonin content higher in the hippocampus and lower in t...

OBJECTIVE To determine placental transfer of ketanserin and to assess the effect of serotonin-2 receptor blockade by ketanserin on serotonin- and phenylephrine-induced vasoconstriction. STUDY DESIGN Five chronically instrumented pregnant ewes at 120 days gestation were injected with 20 mg ketanserin i.v., and fetal and maternal arterial samples were obtained at predetermined intervals to asse...

It is widely believed that damaged axons in the adult mammalian brain have little capacity to regrow, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception, but remain inconclusive. We have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult...

Background: MDMA (3,4-methylenedioxymethamphetamine), which is also known as Ecstasy or Molly, is a commonly found abusive agent in Hong Kong. MDMA abuse is widely reported in electronic dance music (EDM) festivals all over the world. It brings about uncommon mortality and serious morbidity with recreational use, which are believed to be related to serotonin toxicity. Cyproheptadine has anti-hi...