PURPOSE To determine whether keratocytes made fibroblastic in vitro by addition of fetal bovine serum to the medium regain the keratocyte phenotype after culture in serum-free medium. METHODS Collagenase-isolated keratocytes from bovine corneas were plated in DMEM/F-12 containing 1% horse plasma, to allow cell attachment, and then cultured until day 4 in either DMEM/F-12 alone, to retain the ...

The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a so...

PURPOSE To investigate the use of a multistep serum-free culture system in developing a conjunctival epithelial equivalent with improved in vitro and in vivo proliferative properties and to evaluate the effect of serum supplementation and culture conditions on the proliferative capacity of these cells. METHODS Conjunctival epithelial cells were cultivated on human amniotic membrane (HAM) in a...

The effects of epidermal growth factor (EGF), insulin, and a transforming growth factor from fetal bovine serum on protein synthesis, DNA synthesis, and morphology in confluent cultures of AKR-2B mouse cells have been examined. The cells were plated and grown to confluence in serum-containing medium, and then the medium was changed to MCDB 402 containing no undefined supplements for 2 days befo...

The effects of epidermal growth factor (EGF), insulin, and a transforming growth factor from fetal bovine serum on protein synthesis, DNA synthesis, and morphology in confluent cultures of AKR-2B mouse cells have been examined. The cells were plated and grown to confluence in serum-containing medium, and then the medium was changed to MCDB 402 containing no undefined supplements for 2 days befo...

Cultures of chicken embryo fibroblasts infected with the temperature-sensitive transformation mutant of Rous sarcoma virus, tsLA24PR-A, were arrested between mitosis and S phase by exposure to serum-free medium at the non-permissive temperature (41degree C) for 2 days. On shifting to the permissive temperature (35degree C) the cells assumed a transformed morphology and increased uptake of [2min...

U-937 is a human monocytic cell line that has been to elaborate factors that affect normal human hematopoiesis in vitro. Studies on the effects of these factors demonstrated an erythroid potentiating factor (EPF) and a potent inhibitor of granulocyte-macrophage (CFU-GM) colony growth. The EPF was present in both serum-containing and serum-free U-937 conditioned media, had a dose-dependent effec...

Alveolar macrophages (AM) from pathogen-free rabbits were unable to release reactive oxygen intermediates (ROI) unless they were conditioned in serum for 24-48 h before triggering with membrane-active agents. The degree of serum conditioning of AM depended upon the concentration of serum used; optimal ROI release was obtained at or above 7.5% fetal bovine serum (FBS). FBS, autologous rabbit ser...