Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated from Saussurea lappa roots, which had exhibited a wide range of biological activities such as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed counter-current chromatography (HSCCC) was applied for the rapid preparative isolation of sesquiterpenes lactones from <...