Since 1995, new virulent strains of Vibrio parahaemolyticus have emerged and spread throughout the world. These "pandemic" strains have four strain specific genomic islands (GIs), which are considered to be potential factors of the pandemicity. We investigated the origin and function of 24 genes in the so-called VPaI-1, one of the four GIs, by searching homologs in various species in Bacteria a...

Although oxygen has been reported to regulate biofilm formation by several Shewanella species, the exact regulatory mechanism mostly remains unclear. Here, we identify a direct oxygen-sensing diguanylate cyclase (DosD) and reveal its regulatory role in biofilm formation by Shewanella putrefaciens CN32 under aerobic conditions. In vitro and in vivo analyses revealed that the activity of DosD cul...

A significant increase (ca. 22-fold) in the electricity generation due to a Shewanella oneidensis MR-1 biofilm was observed in the presence of Fe(3)O(4)/Au nanocomposites.

Shewanella sp. ECSMB14101 was isolated from marine biofilms formed on the East China Sea. The draft genome sequence comprises 4,272,451 bp with a G+C content of 49.82%. Information on this draft genome will contribute to the understanding of bacterium-animal interactions.

Low solubility of proteins overexpressed in E. coli is a frequent problem in high-throughput structural genomics. To improve solubility of proteins from mesophilic Shewanella oneidensis MR-1 and thermophilic Clostridium thermocellum JW20, an approach was attempted that included a fusion of the target protein to a maltose-binding protein (MBP) and a decrease of induction temperature. The MBP was...

Background: The aim of this study was to investigate antimicrobial effect of Zataria multiflora Boiss essential oil (EO) against six fish spoilage bacteria for evaluation of its potential utilization in the preservation of minimally processed fish products. Methods: Firstly, GC-MS analysis of the EO was performed to determine its chemical composition. Then, antibacterial effect of the EO in a ...

Quantifying the binding energy in DNA-protein interactions is of critical importance to understand transcriptional regulation. Based on a simple computational model, this study describes a high-throughput percentage-of-binding strategy to measure the binding energy in DNA-protein interactions between the Shewanella oneidensis ArcA two-component transcription factor protein and a systematic set ...

Shewanella sp. HN-41 was exploited for selenium nanoparticles synthesis from aqueous selenite compounds under anaerobic conditions. Various reaction conditions, including reaction time, initial biomass, and initial selenite concentration, were systematically investigated to determine their effects on particle size distribution and formation rate. The biomass concentration of Shewanella sp. HN-4...

Soluble manganese in the intermediate +III oxidation state (Mn3+ ) is a newly identified oxidant in anoxic environments, whereas acetate is a naturally abundant substrate that fuels microbial activity. Microbial populations coupling anaerobic acetate oxidation to Mn3+ reduction, however, have yet to be identified. We isolated a Shewanella strain capable of oxidizing acetate anaerobically with M...

This research aims is the agroindustrial wastewater treatment by an electrochemical device called Microbial Fuel Cell (MFC), which produces electricity while to metabolize the wastewater. The behavior of biofilme formed at reticulated carbon vitreous/Shewanella putrefaciens/vinasse solution interface as anode, was studied by electrochemical measurements. The polarization curves against the carb...