A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

Rhododendron decorum is a woody species with high ornamental and medical value. Herein, we introduce novel in vitro regeneration method for R. decorum. We used flower buds to develop an efficient rapid plant protocol. Sterile of 2 cm size were as explants study the effects culture medium growth regulators on callus induction adventitious shoot differentiation, proliferation, rooting. According ...

This research aimed to explore the effect of zeatin (6-γ,γ-dimethylallyl-amino) concentration on in vitro shoot proliferation six olive (Olea europaea L.) cultivars, ‘Aggizi Shami,’ ‘Coratina,’ ‘Frantoio,’ ‘Manzanillo,’ ‘Picual,’ and ‘Toffahi.’ The cultivars were reproduced via multiplication Rugini medium (OM) with three concentrations (2, 4, 6 mg L-1) for subcultures. Shami’ ‘Toffahi’ had hig...

Leaf primordia form around the shoot apical meristem, which consists of indeterminate stem cells. Upon initiation of leaf development, adaxial-abaxial patterning is crucial for appropriate lateral expansion, via cellular proliferation, and the formation of flat symmetric leaves. Many genes that specify such patterning have been identified, but regulation by upstream factors of the expression of...

Interactive effect of plant growth regulators 6-Benzylaminopurine (BAP) (0, 2, 4 and 8 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #23...

Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. Using 40% sodium hypochlorite solution for 40 min, cont...

Many aspects of plant development are associated with changing concentrations of the phytohormone auxin. Several stages of root formation exhibit extreme sensitivities to exogenous auxin and are correlated with shifts in endogenous auxin concentration. In an effort to elucidate mechanisms regulating development of adventitious roots, an ethyl methanesulfonate-mutagenized M2 population of Arabid...

We report about ERF BUD ENHANCER (EBE; At5g61890), a transcription factor that affects cell proliferation as well as axillary bud outgrowth and shoot branching in Arabidopsis (Arabidopsis thaliana). EBE encodes a member of the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor superfamily; the gene is strongly expressed in proliferating cells and is rapidly and transiently up-regu...

We report about ERF BUD ENHANCER (EBE; At5g61890), a transcription factor that affects cell proliferation as well as axillary bud outgrowth and shoot branching in Arabidopsis (Arabidopsis thaliana). EBE encodes a member of the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor superfamily; the gene is strongly expressed in proliferating cells and is rapidly and transiently up-regu...