Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l-1) in combinat...

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%)...

Rye is second only to wheat among grains most widely used in the making of bread and is also a very important gene resource for breeding and improvement of wheat and other cereal crops owing to tolerance to abiotic stress factors such as low temperatures, drought and poor soil conditions. However, application of biotechnologies has been limited in rye breeding since it is one of the most recalc...

Background: Worldwidely cultivated, melon is commercially an important fruit crop, as it is in Iran. Objectives: Establishment of an efficient in vitro plant regeneration system plays a pivotal role in the plant transformation, hence, the importance of regeneration protocol for Iranian melon (Cucumis melo L. var. Gorgab) has encouraged us to work on in vitro melon regeneration. Material and Me...

Eclipta alba is a medicinal herb, also known as Bhringraj belonging to family Asteraceae. The plant has hepatoprotective, antimicrobial and hair growth promoting properties. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Eclipta alba from leaf explant was evaluated. The sterilized explants were inoculated on MS medium supplemented with different conce...

Pigeonpea is an important grain legume, however the genetic transformation of pigeonpea has been very challenging to date. In the present study, we describe a simple protocol for plantlet regeneration via multiple shoot induction from 12-16 day old immature embryos of pigeonpea cultivar ICPL 87 which can be used for efficient transformation. By optimizing the culture medium, we have achieved or...

A plant regeneration protocol was devised for Cardiospermum halicacabum by means of aseptically extracted 7 days old hypocotyls forming adventitious shoots on Murashige and Skoog (MS) medium harmonized with 0.7 μM thidiazuron (TDZ) producing a maximum of 18.20 ± 0.98 number of shoots in 94% cultures following 4 weeks. Subsequent subculturing for five passages, on a medium without plant growth r...

An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed ...

A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...