Desferrioxamines E (1), D2 (2), X1 (3), and X2 (4), four macrocyclic N-hydroxy-N-succinyl diamine-based siderophores, were produced efficiently by heterologous expression of a fusion biosynthetic gene cluster. This expression system consisted of three genes (mbsA-C) from marine metagenomic DNA and one gene (dfoC(C)) from the terrestrial bacterium Erwinia amylovora. The first three genes are fun...

The majority of bacterial species do not grow on synthetic media. Many non-growers require growth factors from other bacteria, but the nature of these compounds is largely unknown. We show here that previously uncultured isolates from marine sediment biofilm grow on a Petri dish in the presence of cultured organisms from the same environment. The growth factors produced by one cultured helper s...

Bacterial siderophores are a group of chemically diverse, virulence-associated secondary metabolites whose expression exerts metabolic costs. A combined bacterial genetic and metabolomic approach revealed differential metabolomic impacts associated with biosynthesis of different siderophore structural families. Despite myriad genetic differences, the metabolome of a cheater mutant lacking a sin...

Mycobacterium tuberculosis (M.tb), which requires iron for survival, acquires this element by synthesizing iron-binding molecules known as siderophores and by recruiting a host iron-transport protein, transferrin, to the phagosome. The siderophores extract iron from transferrin and transport it into the bacterium. Here we describe an additional mechanism for iron acquisition, consisting of an M...

Both plant growth-promoting Pseudomonas B10 and its yellow-green, fluorescent iron transport agent (siderophore) pseudobactin enhance potato growth and biologically control certain soil-borne fungal diseases in part by depriving specific root-colonizing endemic microorganisms including phytopathogens of iron(III), thus inhibiting their growth. The present study examines this mode of iron depriv...

Streptomyces species are effective biocontrol agents toward many plant pathogens. These microorganisms well known for producing secondary metabolites, promoting growth and inducing defense mechanisms. In this study, the ability of tomato root-colonizing strains to trigger resistance against Rhizoctonia solani (J.G. Kühn) AG4 was investigated. For goal, we evaluated pattern LOXB PAL1 genes expre...

Streptomyces , the largest genus in Actinobacteria, has been known as a chemically prolific bacterial group producing pharmaceutically important small molecules. Various endeavors have made to discover novel secondary metabolites from strains inhabiting diverse environmental niches. In our course of collecting biologically active molecules, marine-derived sp. V17-9 was isolated seagrass collect...