Cotinus coggygria Scop. (Anacardiaceae) is a deciduous shrub or small tree that is native to a large area covering from southern Europe, east across central Asia, and the Himalayas in northern China. Shotgun 454 pyrosequencing was used to develop microsatellite markers from the genome of C. coggygria. In this study, 349 microsatellite loci were identified from 40,074 individual sequence reads p...

Mimosa scabrella Benth., popularly known as ''bracatinga'', is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SSR development, when there is no genome sequence, is time and labor intensive and there are no...

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs were evaluated in silico with respect to the SILVA 16S/18S rDNA non-redundant reference dataset (S...

DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three ampl...

We propose a model for the production and suppression of J/ψ mesons in high-energy hadronic collisions. We factorized the process into a production of the cc̄ pairs of relative momentum k = kc− kc̄ convoluted with a transition probability from the produced cc̄ pairs into the observed J/ψ mesons. As the produced cc̄ pairs exit the nuclear matter, the multiple scattering between the colored c and c̄ a...

Source/description: Horse genomic DNA was digested with the restriction enzyme MboI, size selected by gel electrophoresis for fragments between 200 and 1200 bp, and ligated into the BamHI site of the M13 phage vector. Clones containing a potential microsatellite were identified by screening the library with [P] 5¢ end-labelled oligo [dCA]16 and oligo [dGT]16 probes. The DNA was isolated from po...

Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. "Universal" and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to the degree of specificity of primer pairs to different prokaryotic groups. In a reassessment of the published primers commonly used for "universal"...

DNA identity is highly effective and efficient for distinguishing crop varieties regardless of their phenotypic similarities. To establish DNA identity in ramie, 21 simple sequence repeat primers were amplified in 108 accessions of domestic and exotic ramie germplasms. Sixty polymorphic bands were obtained, with an average of 2.9 bands per locus and 2-8 band types per primer locus (average of 5...

The ability to select short DNA oligonucleotide sequences capable of binding solely to their intended target is of great importance in developing nucleic acid based detection technologies. Applications such as multiplex PCR rely on primers binding to unique regions in a genome. Competing side reactions with other primer pairs or template DNA decrease PCR efficiency: Freely available primer desi...

Two new lead(II) complexes [Pb(pcih)(bha)] (1) and [Pb(pcih)(NO3)] (2, Hpcih = 1,4-bis(3-pyridyl)-2,3-diazo-1,3-butadiene, Hbha = 2-hydroxybenzoic acid) have been synthesized by solvothermal methods and structurally characterized. The single-crystal X-ray data show that the coordination spheres of the Pb2+ ions in 1 and 2 are hemidirected with stereochemically active electron lone pairs. There ...