In this study, two antioxidative substances, a homogeneous polysaccharide [abalone shell polysaccharide (ASP-1), corresponding to the first peak by size exclusion chromatography] and a non-polysaccharide compound [abalone shell compound (ACS-2), corresponding to the second peak by size exclusion chromatography], were extracted from the abalone (Haliotis discus hannai Ino) shell. We primarily fo...

The linear peptide gramicidin forms prototypical ion channels specific for monovalent cations and has been used extensively to study the organization, dynamics, and function of membrane-spanning channels. The tryptophan residues in gramicidin channels are crucial for maintaining the structure and function of the channel. We explored the structural basis for the reduction in channel conductance ...

The use of chromatographic stationary phases or solvent modifiers to modulate diffusion properties in NMR experiments is now well established. Their use can be to improve resolution in the diffusion domain or to provide an insight into analyte-modifier interactions and hence the chromatography process. Here we extend previous work using size-exclusion chromatographic (SEC) stationary phases to ...

Abbreviations: mAbs: Monoclonal Antibodies; CQA: Critical Quality Attribute; CDRs: Complementary Determining Regions; FC: Fragment Crystallizable; FAB: Fragment Antigen-Binding; SEC: Size-Exclusion Chromatography; SDS-PAGE: Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis; CE: Capillary Electrophoresis; IEX: Ion Exchange Chromatography; RPC: Reversed Phase Chromatography; MS: Mass Spect...

High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable colum...

Methods Recombinant Pla l 1.0101 was heterologously expressed in the E. coli strain Rosetta-gami B pLysS and purified using cation exchange and size exclusion chromatography. Natural Pla l 1 was obtained by pollen extraction and cation exchange chromatography. Physico-chemical properties of the purified proteins were analyzed in gel electrophoresis, mass spectrometry and circular dichroism. Usi...

The newest achievements in the application of miscellaneous liquid chromatographic techniques such as size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography, and thin-layer chromatography for the elucidation of the various aspects of the binding of ligands to proteins are compiled and briefly discussed. Examples of employment in pharmaceutical and clinical chemis...