OBJECTIVES/HYPOTHESIS The objective was to determine the agreement of the positive results from a multiple skin prick test (SPT) device with the ability to determine a definable endpoint through intradermal dilutional testing (IDT) to compare semiquantitatively the degree of positivity of SPT results with quantitative results from IDT and to analyze the cost of immunotherapy based on SPT compar...

BACKGROUND : The variability of skin prick test results when carried out by multiple users has not previously been assessed across different devices or between different sites on the body. Such multiuser variability has important implications for clinical practice. OBJECTIVES : We assessed the variability of measurements from 4 commonly used single-headed skin test devices when used by multip...

To elucidate the IgE binding site of mugwort (Artemisia vulgaris r.) pollen, pollen grains were frozen and fixed using a cryocut. They were incubated with antibodies according to the following sequence: Sera pool of individuals who showed mugwort-RAST class 3 or 4, biotin-labeled goat anti-human IgE antibody, streptavidin-peroxidase and diaminobenzidine. Then, they were observed under electron ...

Background Skin prick testing and serum food-specific IgE testing are the most commonly used diagnostic tests in evaluating IgE-mediated food reactions. However, the presence of negative tests can be falsely re-assuring such outcomes do not always exclude allergy. Food challenges are therefore indicated. Double-blind, placebo-controlled food challenge is the gold standard for diagnosis, but in ...

Background Exercise-induced anaphylaxis is a rare disorder characterized by the development of a severe allergic response occurring after mildto-strenuous physical activity. Food-dependent exercise-induced anaphylaxis (FDEIA) comprises 30-50% of all cases of exercise-induced anaphylaxis [2]. Diagnosis involves careful history and appropriate skin prick testing and food specific IgE levels [4,6]...

Background. Date palm's pollen has been identified as a source of allergy; the rate of sensitization of this pollen is between 6 and 29%. Objective. To determine the prevalence of sensitization to date palm in Marrakesh and to identify the clinical profile. Patients and Methods. This study is based on a questionnaire and the prick test on 7 allergens, in population aged 5 years and above with c...

The relative importance of atopy in the aetiology of rhinitis is largely unknown. The present study investigated the geographical variations in rhinitis in relation to atopy. The cross-sectional study involved 54,178 children (aged 8-12 yrs) from 30 study centres in 22 countries worldwide. Symptoms of rhinoconjunctivitis and rhinitis without conjunctivitis in the last 12 months were reported in...

BACKGROUND Exposure to cockroach allergen is prevalent in inner-city homes and is associated with an increased risk of cockroach sensitization. OBJECTIVE We sought to determine the prevalence of cockroach allergen exposure in suburban middle-class homes and to study its relationship to cockroach sensitization. METHODS Children with asthma, 6 to 17 years of age, were recruited from 3 pediatr...

BACKGROUND The aim of the study was to evaluate the relationship between house dust mite, cat and dog allergen levels with household characteristics in the houses of children living in urban and rural areas in central Poland. METHODS Dust samples were collected from 141 urban and 191 rural houses. Der f1 + Der p1, Can f 1, and Fel d1 levels were measured and associated with residential condit...

background: the prevalence of allergic diseases has risen in the last decades. the objective of this study was to determine the common allergens in children via the skin prick test. methods: this cross-sectional study recruited 313 allergic children (4 months to 18 years old) referred to the asthma and allergy clinic of children’s medical center in tehran. a questionnaire containing demographic...