Homozygote individuals (HO) of the GH-transgenic zebrafish lineage (F0104), despite expressing double the amount of growth hormone (GH) in relation to the hemizygote (HE) individuals, presented smaller growth in relation to the last, and similar to the non-transgenic (NT) group. Through the analysis of the expression of genes of the somatotrophic axis in the livers of HO and NT individuals, it ...

Cytokines regulate important cellular responses through signal transduction pathways. The computer modeling is a useful method to understand dynamic characteristics or regulatory mechanisms of signal transduction pathways. The ultrasensitivity of the mitogen-activated protein kinase cascade was shown by the simulation study [2]. Many cytokine receptors transduce signals by receptor bound JAK ki...

Although elevation of the blood glucose level is a causal adverse effect of treatment with interferon (IFN), the precise underlying molecular mechanism is largely unknown. We examined the effects of type I and type II IFN (IFN-β and IFN-γ) on insulin-induced metabolic signaling leading to glucose uptake in 3T3-L1 adipocytes. IFN-β suppressed insulin-induced tyrosine phosphorylation of IRS-1 wit...

The life strategy of the anadromous Arctic charr (Salvelinus alpinus) includes several months of voluntary fasting during overwintering in freshwater, leading to emaciation prior to seawater migration in spring. In this study we compared changes in condition, substrate utilization and liver metabolism between captive anadromous charr subjected to food deprivation during late winter and spring, ...

Deoxynivalenol (DON), a trichothecene mycotoxin found in grains and cereal-based foods worldwide, impairs weight gain in experimental animals but the underlying mechanisms remain undetermined. Oral exposure to DON induces rapid and transient upregulation of proinflammatory cytokine expression in the mouse. The latter are known to induce several suppressors of cytokine signaling (SOCS), some of ...

OBJECTIVE Suppressors of cytokine signaling (SOCS) proteins are intracellular regulators of receptor signal transduction, mainly Janus kinase/signal transducers and activators of transcription (JAK/STAT). We investigated the effects of SOCS modulation on the JAK/STAT-dependent responses in vascular cells, and their implication in atherosclerotic plaque development. METHODS AND RESULTS Immunoh...

MicroRNAs (miRNAs) are noncoding RNA molecules that play a significant role in atherosclerosis pathogenesis through post-transcriptional regulation. In the present work, bioinformatic analysis using TargetScan and miRdB databases was performed to identify miRNAs targeting three genes involved foam cell (CD36, Vav3, SOCS1). A total number of hundred sixty-seven were recognized only miR-155–5p se...

Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Accumulated evidences suggest that microRNAs (miRNAs) are related with inflammation-associated diseases. The aim of this study is to investigate whether miR-155 is involved in lipopolysaccharide (LPS)-induced kidney injury, and to explore the underlying mechanisms. Mice were intraperiton...

BACKGROUND Suppressor of cytokine signaling genes (SOCS) are regarded as pivotal negative feedback regulators of cytokine signals, including the interferon-gamma (IFN-γ), granulocyte-colony stimulating factor, and interleukin families, released by T cells. A detailed understanding of the involvement of SOCS genes in graft-versus-host disease (GVHD) is critical to effectively manage GVHD, yet th...