BACKGROUND Busulfan (Bu) requires therapeutic drug monitoring (TDM) in subjects undergoing a conditioning regimen for hematopoietic stem cell transplantation (HSCT). To speed up the procedure and increase reproducibility, we improved our routine LC-MS/MS assay using the on-line solid-phase extraction (SPE) of samples. METHODS A protein precipitation (PP) step was performed before the on-line ...

in this work, a new, simple and fast method for the solid phase extraction-spectrophotometric determination of phosphate using cetyltrimethyl ammonium bromide immobilized on alumina-coated magnetite nanoparticles (ctab@acmnps) has been developed. the determination of phosphate is based on the molybdenum blue method which was monitored at λmax of 810 nm. mnps and acmnps characterized by sem, vsm...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (

background and objectives: the addition of plasticizers (mainly phthalates and adipates) makes plastic flexible and popular for packaging, storing and preserving food, water, and so on. the most widely used plasticizer in food contact applications is di-ethylhexyl phthalate (dehp) though being suspected to have carcinogenic and estrogenic properties. the aim of this study is application of magn...

analytical chemistry has experienced, as well as other areas of science, a big change due to the needs and opportunities provided by analytical nanoscience and nanotechnology. now, nanotechnology is increasingly proving to be a powerful ally of analytical chemistry to achieve its objectives, and to simplify analytical processes. moreover, the information needs arising from the growing nanotechn...

this article presents the method of extraction-preconcentration of lead, cadmium, and nickel ions from food samples using the ghezeljeh montmorillonite nanoclay (geleh-sar-shoor) as a new native adsorbent in batch single component systems. the extraction-preconcentration of heavy metals were carried out by applying the solid phase extraction (spe) method followed by atomic absorption spectrosco...

a novel, magnetic silica sorbent with polyelectrolyte multilayers (pems) on its surface was prepared, and was used for magnetic solid phase extraction (mspe) of trace a+ via flame atomic absorption spectrometry (faas). the experimental parameters for the mspe procedure, such as the ph, type, and concentration of eluent, ultrasonic time and effects of co-existing ions were investigated. the dete...

a simple, rapid and sensitive solvent assisted-dispersive solid phase extraction method was developed for the extraction of iron(ii) prior to its spectrophotometric determination. the fe(ii) reacted with 2,4,6-tris(2-pyridyl)-1,3,5-triazine, neutralized with sodium dodecyl sulfate and extracted onto the fine particles of benzophenone which were formed upon rapid injection of a mixture of benzop...

Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, ...

We describe a simple, specific, and sensitive reversed-phase HPLC method with automated solid-phase extraction (SPE) for analyzing alprazolam, clonazepam, and nitrazepam concentrations in human serum or plasma. We prepare samples and calibrators with an automated sample preparer, using 100-mg Bond-Elut C18 SPE columns. The HPLC method uses isocratic elution with acetonitrile:methanol:dipotassiu...