Recent advances in somatic cell reprogramming have highlighted the plasticity of the somatic epigenome, particularly through demonstrations of direct lineage reprogramming of one somatic cell type to another by defined factors. However, it is not clear to what extent this type of reprogramming is able to generate fully functional differentiated cells. In addition, the activity of the reprogramm...

Currently, somatic cell count (SCC) and bacterial culture are considered as the gold standard of detecting subclinical Mastitis. Mastitis leads to proliferation of lymphocytes in the supramammary lymph nodes and subsequent enlargement of ipsilateral lymph node. Ultrasonography can be used to survey these changes. A portable ultrasound machine with a 2-5 MHz convex transducer was used to identif...

Testicular function and future fertility may be affected by cancer treatment during childhood. Whilst survival of the germ (stem) cells is critical for ensuring the potential for fertility in these patients, the somatic cell populations also play a crucial role in providing a suitable environment to support germ cell maintenance and subsequent development. Regulation of the spermatogonial germ-...

Background: Somatic cell nuclear transfer (SCNT) provides an appealing alternative for the conservation and improvement of genetic material of unique and endangered species. An important prerequisite for successful SCNT is the availability of good quality donor cells, as normal embryo development is dependent upon proper reprogramming of the donor genome so that embryonic genes can be appropria...

Progress on industrial-scale propagation of conifers by somatic embryogenesis has been hampered by the differences in developmental capabilities between cell lines, which are limiting the capture of genetic gains from breeding programs. In this study, we investigated the metabolic events occurring during somatic embryo development in Norway spruce to establish a better understanding of the fund...

Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture coffea plant in area over 40.000ha South Good quality seedlings have a big demand. Using bioreactor technique the first method resolve lack seedlings. Stem, leaves vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supple...

Abstract To determine the optimal embryogenic capacity (somatic embryo production) of selected elite nematode-resistant genotypes Pinus thunbergii , variables such as tissue (ET) morphology, maternal genotype, proliferation rate and age were analyzed. ET morphology histological evaluation stage showed a decrease in filamentous clump protuberant surfaces decline acetocarmine-staining area, which...

Reprogramming of somatic cells to different extents has been reported using different methods. However, this is normally accompanied by the use of exogenous materials, and the overall reprogramming efficiency has been low. Chemicals and small molecules have been used to improve the reprogramming process during somatic cell nuclear transfer (SCNT) and induced pluripotent stem (iPS) cell generati...

abstract the objective of this study was to determine the effect of psychrotrophic bacteria and somatic cells on the physicochemical characteristics of iranian ultrafiltered (uf) white cheese. milks with different numbers of psychrotrophic bacteria (1×106, 5×106 and 10×106 cfu/ml) and low somatic cell counts (scc, <2×105/ml) were selected and uf white cheese trials were produced. in a separate ...

This article proposes the concept of genome network, describes different variations of the somatic genome network, and reviews the agricultural implications of such variations. All genetic materials in a cell constitute the genome network of the cell and can jointly influence the cell's function and fate. The somatic genome of a plant is the genome network of cells in somatic tissues and of non...