The distribution of rat liver epoxide hydrolase in various subcellular fractions was investigated by immuno-electron-microscopy. Ferritin-linked monospecific anti-(epoxide hydrolase) immunoglobulins bound specifically to the cytoplasmic surfaces of total microsomal preparations and smooth and rough microsomal fractions as well as the nuclear envelope. Specific binding was not observed when the ...

Radionuclide therapy requires patient-specific planning of the absorbed dose to target volumes, in most cases tumours, in order to achieve an expected biological effect, taking into account that the absorbed doses to normal organs and tissues should be kept as low as reasonably achievable. Therefore, the calculation of absorbed doses has to be as accurate as possible. The accuracy depends on th...

background snake venom is a complex mixture of different components including proteins and peptides that mostly has enzymatic properties. in this study, the alkaline phosphatase (alp) activity is measured in iranian vipera lebetina, venomous snake on sephadex g-100 to investigate the suitability of this procedure. material and methods the method of this study is experimental. 100 mg of the crud...

introduction: iranian scorpion species are classified in buthidae and scorpionidae with 16 genera and 25 species. in iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming. mesobuthus eupeus is the most common species in iran. its venom contains several toxin fractions which can affect the ion channel. in this study purification, ...

Background: HDR brachytherapy is one of the commonest methods of nasopharyngeal cancer treatment. In this method, depending on how advanced one tumor is, 2 to 6 Gy dose as intracavitary brachytherapy is prescribed. Due to high dose rate and tumor location, accuracy evaluation of treatment planning system (TPS) is particularly important. Common methods used in TPS dosimetry are based on computat...

1. Subcellular fractions, characterized by using morphological, compositional and enzymic markers, were prepared from rat heart tissue and cells isolated from the hearts of fed and 24h-starved rats. 2. The lipoprotein lipase activity of fractions from whole tissue and isolated cells was determined in either fresh fractions or in acetone/diethyl ether powders of the fractions. 3. Lipoprotein lip...

Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies ca...

Soluble factors appear to be present in both nuclei and cytoplasm of Krebs 2 ascites tumour cells capable of stimulating the enzyme catalysed methylation of DNA in isolated nuclei from these cells.

We obtained cell preparations containing greater than 95% neutrophils from freshly drawn bovine blood. The cells were suspended in sucrose and disrupted in a Dounce homogenizer, and the postnuclear supernate was fractionated by zonal differential sedimentation and by isopycnic equilibration. The subcellular fractions were characterized biochemically by testing for marker enzymes and other const...

The relationship between polyamines and RNA synthesis was studied by considering the action of spermine on histone acetylation in perfused heart. In addition, the effect of methylglyoxal bis(guanylhydrazone), inhibitor of putrescine-activated S-adenosylmethionine decarboxylase activity, on RNA and polyamine specific radioactivity and on acetylation of histone fractions was also investigated in ...