8-Methoxy psoralen (8-MOP) exerts a short-term (24 h) mitogenic action, and a long-term (48-72 h) anti-proliferative and melanogenic action on two human melanoma cell lines, SK-Mel 28 and C32TG. An increase of intracellular calcium concentration was observed by spectrofluorometry immediately after the addition of 0.1 mM 8-MOP to both cell lines, previously incubated with calcium probe fluo-3 AM...

Elevated cytosolic free calcium (Ca(i)) and reciprocally reduced, extracellular ionized calcium (Ca-ion) levels are observed in both hypertension and non-insulin-dependent diabetes mellitus (NIDDM). Because the changes of vascular function and insulin sensitivity in these conditions resemble the changes associated with "normal" aging, we wondered to what extent similar alterations in calcium me...

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with mot...

  ABSTRACTBackground : In order to quantify effects of ultrasound irradiation parameters under therapeutic condition, especially sonodynamic therapy, it is initially necessary to evaluate inertial cavitation activity in vitro conditions therefore, in this study, the effect of 1 MHz low level ultrasound based on °OH radicals generated by acoustic inertial cavitation in aqueous solution was monit...

abstractbackground : in order to quantify effects of ultrasound irradiation parameters under therapeutic condition, especially sonodynamic therapy, it is initially necessary to evaluate inertial cavitation activity in vitro conditions therefore, in this study, the effect of 1 mhz low level ultrasound based on °oh radicals generated by acoustic inertial cavitation in aqueous solution was monitor...

BACKGROUND Since estrogen is known to increase vascular endothelial cell growth, elevated estrogen exposure from hormone replacement therapy or oral contraceptives has the potential to contribute in the development of abnormal proliferative vascular lesions and subsequent thickening of the vasculature. How estrogen may support or promote vascular lesions is not clear. We have examined in this s...