In the present research we have studied the distribution and the changes of the oligosaccharidic component of the glycoconjugates during the development of the choroids plexuses of the 3rd ventricle and of the lateral ventricles in chick embryos and in 3-day-old chicken. For this purpose a battery of seven horseradish peroxidase labelled lectins was used. Lectin histochemistry has shown that st...

Introduction: Alteration of terminal sugars of Glycoconjugates is one of the important aspects of neoplasia. The aim of the present study was to identify GalNac and GlcNac containing glycoconjugates in different grades of neoplastic cells in adenocarcinoma of colon.  Methods: Histological sections prepared from paraffin blocks belong to 30 patient of colon adenocarcinoma obtained from Khatam...

Background: Cryopreservation has adverse effect on human spermatozoa via exposure to physical and chemical stress that may lead to alter in reactive oxygen species (ROS) levels. During sperm preparation or cryopreservation, centrifugation as a physical stress may effect on sperm quality probably with stress oxidative production and membrane damage. Therefore the aim of this study was to evaluat...

The carbohydrate specificity of three novel lectins, Boletopsis leucomelas lectin (BLL), Aralia cordate lectin (ACL), and Wasabia japonica lectin (WJL), was examined by frontal affinity chromatography using a panel of fluorescently labeled 47 oligosaccharides. The results indicate that BLL recognizes an agalacto structure of the biantennary chain and its bisecting structure. ACL showed strong a...

BACKGROUND Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been de- scribed for activation of a temporary response in ooc...

Glycoconjugates in secretory epithelium of the anterior lingual salivary glands of chicken have been studied by means of light microscopic histochemical methods. A series of staining procedures were employed including peroxidase labeled lectin diaminobenzidine methods and digestion techniques with neuraminidase or α amylase. The terminal portion of the anterior lingual gland consisted of mucous...

The ciliary base is marked by a transition zone in which Y-shaped cross-linkers extend from doublet microtubules to the plasma membrane. Our goal was to investigate the hypothesis that the cross-linkers form a stable interaction between membrane or cell surface components and the underlying microtubule cytoskeleton. We have combined Triton X-100 extraction with lectin cytochemistry in the photo...

The laboratory rat, Rattus norvegicus, was the first mammalian species domesticated for scientific research, which work dating back to before 1850. From this auspicious beginning, the rat has become the most widely studied experimental animal model for biomedical research (Jacob, 1999). Since the development of the first inbred rat strain by King 1909, over 500 inbred rat strains have been deve...

Sperm cryopreservation is the best modality to ensure future fertility for males diagnosed with cancer. The extent to which cryopreserved sperm is actually used for impregnation, the fertility treatment options that are available and the success rates of these treatments have not been investigated in depth. The medical records of 682 patients who cryopreserved sperm cells due to cancer treatmen...

Introduction It is well known that cryopreserved semen could be used to regenerate commercial or research poultry lines; however, fertility rates from poultry semen frozen with current methods are not reliable enough for germ-line retrieval, especially from lines with low reproductive efficiency. As part of a long-term research plan to determine how and why poultry sperm lose functional compete...