Fixation of cat stellate ganglia by perfusion in situ with cold 4% formaldehyde in Krebs-Ringer solution, followed by immersion in the same solution for 3 or 6 hr, produced inactivation of approximately one-half the acetylcholinesterase (AChE) and butyrocholinesterase (BuChE); after 21 hr, further inactivation occurred. Similar fixation with glutaraldehyde (1, 2 and 4%) inactivated over three-q...
