UNLABELLED : Human embryonic stem cells (hESCs) have an enormous potential as a source for cell replacement therapies, tissue engineering, and in vitro toxicology applications. The lack of standardized and robust bioprocesses for hESC expansion has hindered the application of hESCs and their derivatives in clinical settings. We developed a robust and well-characterized bioprocess for hESC expan...

Bacterial pre-oxidation of refractory gold concentrates generates large volumes leachate and requires a significant supply nutrients to support bacterial growth. Therefore, bioleachate reuse reduces both water consumption the required for process. However, efficiency this method its benefit need be further explored. In present study, two tests on in new cycles oxidation were carried out evaluat...

Pseudomonas putida KT2440 is an emerging biomanufacturing host amenable for use with renewable carbon streams including aromatics such as para-coumarate. We used a pooled transposon library disrupting nearly all (4,778) non-essential genes to characterize this microbe under common stirred-tank bioreactor parameters quantitative fitness assays. Assessing differential values by monitoring changes...

The main bottleneck in fermentation technology is scaling up procedure of industrial enzymes according to the biological characteristics organism. current study describes production kinetics cellulases stirred tank reactor by using mutant and wild strains T. dupontii. span both bioreactor was examined. It found strain dupontii time required for optimum reduced 48h as compared 72h strain. kineti...

BACKGROUND For the cultivation of Escherichia coli in bioreactors trace element solutions are generally designed for optimal growth under aerobic conditions. They do normally not contain selenium and nickel. Molybdenum is only contained in few of them. These elements are part of the formate hydrogen lyase (FHL) complex which is induced under anaerobic conditions. As it is generally known that o...