Objective To produce two supplemented agar types in order to store pneumococci for several months at room temperature. Methods Todd-Hewitt/Hemoglobin/Yeast/Charcoal/Agar (TH-HYC) and Todd-Hewitt/Skim-Milk/Yeast/Charcoal/Agar (TH-SYC) were used to prepare two supplemented agar types. Nineteen pneumococci isolated from patients or asymptomatic carriers displaying diverse serotypes and multilocu...

In 1910 Carrel and Burrows reported the successful cultivation in vitro of a human sarcoma. They used as a culture medium plasma from the patient from whom the tumor was removed, and observed an active migration of cells during several days' incubation. No subcultures were made. In subsequent attempts to cultivate human tissue Carrel experienced considerable difficulty owing apparently to the l...

Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM culture media with a pH range from 6...

Bone marrow stromal cells are a mixed population that contribute to the formation of the hematopoietic microenvironment. The osteogenic lineage includes populations of cells that, in culture, form discrete nodules of mineralized tissue when grown in the presence of ascorbic acid and sodium beta-glycerophosphate. We have used nodule formation to assay for the self-renewal capacity of osteoprogen...

Intraspecific fusion of protoplasts with nuclei in Antrodia cinnamomea lengthened the time for development of colonies on regeneration medium and reduced the number of protoplasts capable of regeneration. When 2.1 × 10 C98 protoplasts were fused with C96 nuclei, 1750 colonies appeared on the regeneration medium, and 742 of them were putative nuclear hybrids as their colonies were different from...

To determine the need for blind subculturing of BACTEC (Johnston Laboratories, Cockeysville, Md.) blood culture media, we compared results of radiometric readings, visual inspection, and blind subculturing for nearly 7,500 blood specimens. Visual inspection and radiometric testing were performed on day 1 through 7, and blind subcultures were made on day 3. In the first phase of the study, 402 o...

PURPOSE A successful method to subculture human lens epithelial (HLE) cells that retain their intrinsic characteristics is of great importance. This study examines the effects of four different growth factors on proliferation and differentiation in HLE cells in early subcultures. METHODS Specimens of HLE cells were obtained from infants. First- or second-passage cells were cultured in the pre...