This paper reports the possibility of monitoring the sweetener content in the leaves of Stevia Rebaudiana and root extract of Ginseng using FT-IR, micro-Raman and SERS techniques. Fingerprint analysis is a direct, fast, nondestructive and effective method to discriminate and identify different species. Ginseng root contains ginsengoside saponin about 4% and other active compounds such as D-gluc...

Possessing putative hypolipidemic effects, Teucrium polium (TP) have been traditionally used as a medicinal plant in Iran. The aim of the present study was to investigate this effect on the sucrose-induced insulin resistance male rat model. Thirty Wistar male rats weighting 180 ± 20 g were divided into five groups of six each. Four groups were given sucrose 50% in drinking water for 10 weeks. I...

Sucrose synthase (SuSy) is considered the first key enzyme for secondary growth because it is a highly regulated cytosolic enzyme that catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. Although SuSy enzymes preferentially functions in the direction of sucrose cleavage at most cellular condition, they also catalyze the synthetic reaction. We isolated a gene th...

Possessing putative hypolipidemic effects, Teucrium polium (TP) have been traditionally used as a medicinal plant in Iran. The aim of the present study was to investigate this effect on the sucrose-induced insulin resistance male rat model. Thirty Wistar male rats weighting 180 ± 20 g were divided into five groups of six each. Four groups were given sucrose 50% in drinking water for 10 weeks. I...

Contrasting evidence has accumulated regarding the role of acid invertase and sucrose synthase in tomato fruit sink establishment and maintenance. In this work the relationships among the activities of sucrose synthase and acid invertase, Lycopersicon esculentum Mill cv UC-82B fruit growth, and starch accumulation were analyzed in fruit at 0 to 39 d after anthesis. Sucrose synthase, but not aci...

Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity ...

We describe a simple and low cost nonlinear gradient former. The apparatus is derived from a 20 ml syringe and was used to build up sucrose gradients (1) for the separation of DNA fragments needed for the construction of a cosmid library. The setup is given in Figure 1. The lower chamber underneath the plunger is the mixing chamber containing a given volume of the lower concentrated solution, t...

Isoamylases hydrolyse (1-6)-alpha-D-glucosidic linkages in starch and are involved in both starch granule formation and starch degradation. In plants, three isoamylase isoforms with distinct functions in starch synthesis (ISA1 and ISA2) and degradation (ISA3) have been described. Here, we created transgenic potato plants with simultaneously decreased expression of all three isoamylases using a ...

Objective: In this research, the production and quality characteristics of new healthy pomegranate snacks (Punica granatum L.) obtained by drying after different pre-treatments were investigated. Material Methods: Besides control group, three applied to seeds before drying. Pomegranate without any pre-treatment grouped as “control” samples (C) other group was boiled in 80 °C water for 2 minutes...

Aim. Analysis of physiological and biochemical characteristics genetically modified wheat with partially suppressed activity the proline dehydrogenase gene. Methods. Determination (PDH) activity, free L-proline (Pro); carbohydrate content (sucrose fructose). Results. It was established that under conditions water deficit in plants integrated elements forming double-stranded RNA suppressor pdh g...