ABSTRACT: For the DNA-based study of plant species, one important steps is to obtain high-quality DNA. However, this problematic when species contains a lot polyphenols and polysaccharides. The polysaccharides interfere with activity Taq polymerase enzyme during PCR reaction thereby affecting quality Therefore, method for DNA extraction from Cyclanthera pedata has been developed. current reveal...

Structurally and functionally modified nucleotides expand the scope of therapeutic and diagnosticapplications of oligonucleotides. Incorporation of these into DNA and replication of oligonucleotidescontaining them by polymerases may cause pausing, early termination, and misincorporation,however. Reported here is a simple in vitro assay to screen for DNA polymerases that accept modif...

Pfu DNA Polymerase(a)-generated blunt-end PCR fragments can be ligated into the pGEM®-T and pGEM®-T Easy Vectors(b,c) if the fragments are first A-tailed using Taq DNA Polymerase(a). We describe a method for A-tailing these PCR fragments and demonstrate its utility by cloning two different Pfu DNA Polymerase-generated fragments into the pGEM®-T Easy Vector, using the new 2X Rapid Ligation Buffe...

One of the major problems in ancient DNA work is the presence of inhibitory substances, which hampers Taq polymerase activity. Therefore analysis of ancient DNA sample is very challenging. Here we describe a simple and competent ethanol re-precipitation based protocol for the purification of DNA from ancient bones and tissues. The efficiency of this procedure has been demonstrated on 600 years ...

Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...