BackgroundThe pericardial space, which contains fluid (PF), maintains homeostatic conditions that facilitates optimal heart function. The loss of PF during surgery perturbs the equilibrium this environment, contributing to postsurgical complications, including postoperative adhesion formation (PPAF) and atrial fibrillation (POAF), both involve immune-mediated pathways. We have yet fully underst...

Next-generation sequencing (NGS) of human bladder cancer has revealedmany gene alterations compared with normal tissue, with most being predicted to be "loss of function." However, given the high number of alterations, evaluating the functional impact of each is impractical. Here, we develop and use a highthroughput, in vivo strategy to determinewhich alterations are loss of function in tumor g...

(primary vs metastatic tumour). We have shown that the expression of leptin (also considered as a cytokine-like) receptor in colon tumour cells influences chemokine expression depending on the colon location and MMR status. 11 Simultaneous increases in FoxP3, IL17, IL6 and TGFb expression in the majority of sporadic CRC (microsatellite stable (MSS) and leftsided), argue for a disturbed immune r...

Induction of protein kinase C (PKC) pathway in the vascular tissues by hyperglycemia has been associated with many of the cellular changes observed in the complications of diabetes. Recently, we have reported that the use of a novel, orally effective specific inhibitor of PKC b isoform (LY333531) normalized many of the early retinal and renal hemodynamics in rat models of diabetes. In the prese...

BACKGROUND Ovarian follicle growth and maturation requires extensive communication between follicular somatic cells and oocytes. Recently, intercellular cell communication was described involving cell-secreted vesicles called exosomes (50-150 nm), which contain miRNAs and protein, and have been identified in ovarian follicular fluid. The goal of this study was to identify a possible role of exo...