Microfocusing X-rays direct high-density photons on crystal samples and can enhance the diffraction limit quality of collected data. However, these intense cause radiation damage to sample, which often results in undesirable structural information. Accordingly, a data collection strategy that minimizes is critical obtaining accurate In this study, single-point was investigated at two different ...

1. Rodrı́guez A, Matheu V, Trujillo MJ, Martı́nez MI, Baeza ML, Barranco R et al. Grape allergy in paediatric population. Allergy 2004;59:364. 2. Kalogeromitros DC, Makris MP, Gregoriou SG, Mousatou VG, Lyris NG, Tarassi KE et al. Grape anaphylaxis: a study of 11 adult onset cases. Allergy Asthma Proc 2005;26:53– 58. 3. Alcoceba Borràs E, Botey Faraudo E, Gaig Jané P, Bartolomé Zavala B. Alcohol-...

The accuracy of X-ray diffraction data depends on the properties of the crystalline sample and on the performance of the data-collection facility (synchrotron beamline elements, goniostat, detector etc.). However, it is difficult to evaluate the level of performance of the experimental setup from the quality of data sets collected in rotation mode, as various crystal properties such as mosaicit...

Water deficit (WD) in Lupinus albus L. brings about tissue-specific responses that are dependent on stress intensity. Carbohydrate metabolism is very sensitive to changes in plant water status. Six days from withholding water (DAW), sucrose, glucose and fructose levels of the leaf blade had already increased over 5-fold, and the activities of SS and INV(A) had increased c. 1.5-2 times. From 9 D...

Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating biotic stresses affecting the citrus industry. Meiwa kumquat (Fortunella crassifolia) is canker-resistant, while Newhall navel orange (Citrus sinensis Osbeck) is canker-sensitive. To understand the molecular mechanisms underlying the differences in responses to Xcc, transcriptomic profiles of the...

Protein crystallography data collection at synchrotrons is routinely carried out at cryogenic temperatures to mitigate radiation damage. Although damage still takes place at 100 K and below, the immobilization of free radicals increases the lifetime of the crystals by approximately 100-fold. Recent studies have shown that flash-cooling decreases the heterogeneity of the conformational ensemble ...

Along with the adoption of three-dimensional electron diffraction (3D ED/MicroED) as a mainstream tool for structure determination from sub-micron single crystals, questions about best practices regarding each step along workflow, data collection to solutions, arise. In this paper, we discuss three particular aspects 3D ED/MicroED experiment which, after hundreds structures solved in Rigaku’s l...