In a recent edition of the Journal of Clinical Microbiology, Qian et al. described the sensitivity and specificity of the direct tube coagulase (DTC) test for the presumptive identification of Staphylococcus aureus directly from positive blood culture bottles from which Gram stains were consistent with staphylococci (7). The reported sensitivities of 34% at 2 h of incubation and 65% at 4 h of i...

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions degrade pectic substances. In present study, we have isolated Aspergillus niger strain from soil samples, in India and evaluated pectinase production. The highest producing A. was further optimized with various agricultural wastes. Plackett-Burman design (PBD) Central composite ‘(CC...

Abstract. Utami AP, Fahrurrozi, Meryandini A. 2022. Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads. Biodiversitas 23: 3960-3966. Pectinase is one of the enzymes often used in industrial sector, especially food industry, such as extracting clarifying juices. Previous studies have obtained pectinolytic microbial cultures isolated cocoa beans. production pure p...

In this study, complete purification, characterization, and immobilization of polygalacturonase (pectinase) from Penicillium notatum were carried out to achieve an economical suitable alternative for industrial fruit juice clarification. Biosynthesis was under pre-optimized conditions employing solid-state fermentation at a pilot scale using wheat bran. The enzyme subjected series steps purific...

Pectinases are most significant enzymes of commercial sector. Among all the commercially produced industrial enzymes, pectinases occupy 25% share in overall enzyme market. have wide applications various processes such as fruit juice clarification, wastewater remediation and textile paper industry etc. Pectinase is suitable environment friendly tool nature for sustainable development. Although, ...

This work evaluates the enzymatic production of hydrolases and oxireductases by endophytic fungi isolated from leaves caesarweed (Urena lobata L.), which is a perennial plant that well adapted to Amazon floodplains region, being widely used in hessian sacks other products natural fiber. Fungi strains Fusarium sp. (1290) Corynespora (1291) were reactivated, had their DNA extracted sequenced obta...

The goal is to improve the flavors of Arabica coffee by combining semi-carbonic maceration (SCM) process, with enzymatic process at various lengths and temperature. Five different variations SCM processes were investigated: pulped natural (control); 15 oC for 10 days (SCM-15); 20 (SCM-20); 0.01 g.L-1 pectinases A (polygalacturonase, pectin esterase lyase) 5 (SCM-PA); 0.03 B esterase, lyase ß-gl...

Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) usi...