The population of many splendid orchids is reducing from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Asymbiotic seed germination has been applied for ex situ conservation of rare, endangered and threatened orchid taxa and could provide rapid means their multiplication. In the present study reported here, seeds of an epiphytic and rar...

A new approach was established for the regeneration of Trichosanthes kirilowii from root, stem, and leaf explants by somatic embryogenesis (SE), involving a previously unreported SE structure, rhizoid tubers (RTBs). During SE, special rhizoids were first induced from root, stem, and leaf explants with average rhizoid numbers of 62.33, 40.17, and 11.53 per explant, respectively, on Murashige and...

An in vitro regeneration protocol was developed for Capsicum chinense Jacq. cv. Naga King Chili, a very pungent chili cultivar and an important horticultural crop of Nagaland (Northeast India). Maximum number of shoot (13 ± 0.70) was induced with bud-forming capacity (BFC) index of 10.8, by culturing nodal segments in Murashige and Skoog (MS) medium supplemented with 18.16 μM Thidiazuron (TDZ) ...

The cytokinin receptor AHK3 of Arabidopsis thaliana plays a predominant role in shoot development. A study of the hormone-binding characteristics of AHK3 compared with the mainly root-confined receptor CRE1/AHK4 has been accomplished using a live-cell binding assay on transgenic bacteria expressing individual receptor proteins. Both receptors bound trans-zeatin (tZ) with high affinity. Scatchar...

Attempts were made during the 1990 growing season to induce somatic embryos from non-zygotic tissues of five economically important walnut cultivars. The following explants were employed: fertilized and unfertilized ovules; immature and mature catkins; abnormal "spike" flowers; and in vitro-grown leaves and roots. Modifications of the standard induction media were also examined. These included ...

Pterocarpus santalinus belongs to fabaceae family and is one of the endemic and endangered medicinal plant species of seshachalam hills, Tirumala, Andhra Pradesh state, India. In the present study we have successfully demonstrated the in vitro regenartion of this plant through shoot bud culture. The seeds germinated sequentially on plastic trays and transferred polythene bags for 21 days served...

Foliar sprays of growth regulators have commercial potential for improving the performance some parameters associated with alternate bearing in pecan trees. The objective was to evaluate behaviour through analysis seasonal variations buds and leaflets non-structural carbohydrates (glucose, fructose, sucrose, starch), mineral nutrients (N-total, P, K+, Ca2+, Mg2+, Fe2+, Cu2+, Mn2+ Zn2+), yield c...

In this study, several methods have been used to facilitate shoot formation from nodal explants of local almond ecotypes known as “Beldi” grown in Eastern Morocco. Nodal segments divers old genotypes were cultured on various concentrations auxin (indole-3-butyric acid (IBA)) and cytokinins (6-benzyl-aminopurine (BAP), thidiazuron (TDZ), kinetin (KIN)) added two different media (Murashige Skoog ...