At the core of the rhythmically active leech heartbeat central pattern generator are pairs of mutually inhibitory interneurons. Synaptic transmission between these interneurons consists of spike-mediated and graded components, both of which wax and wane on a cycle-by-cycle basis. Low-threshold Ca2+ currents gate the graded component. Ca imaging experiments indicate that these low-threshold curr...

Level shifters play critical roles in ultra low-voltage circuits and systems. a new low power level shifter (LS) is presented for robust logic voltage shifting from near/sub threshold to above threshold domain. The new circuit combines the multi threshold CMOS technique along with novel topological modifications to guarantee a wide voltage conversion range with limited static power and total en...

in present study, a sybr green based real time pcr assay was developed for specific detection and quantification of bacillus subtilis in dough used for bread making. new primer pairs were designed to amplify a 212 base pair fragment of the apre gene. specificity of these primer pairs was confirmed with conventional and real time pcr methods. standard curves constructed using the threshold cycle...

Fluorescent monitoring of DNA amplification is the basis of real-time PCR, from which target DNA concentration can be determined from the fractional cycle at which a threshold amount of amplicon DNA is produced. Absolute quantification can be achieved using a standard curve constructed by amplifying known amounts of target DNA. In this study, the mathematics of quantitative PCR are examined in ...

A Translation Invariance Denoising Algorithm with Wavelet Threshold and its Application on Signal Processing of Laser Interferometer Hydrophone is investigated. The obtained signal of Laser interferometer hydrophone exist a large number of singularity points, and the denoising algorithm of Donoho’s wavelet threshold may produce the Pseudo Gibbs phenomenon on the singularity points. To eliminate...

We study the Maker-Breaker H-game played on the edge set of the random graph G(n, p). In this game, two players, Maker and Breaker, alternately claim unclaimed edges of G(n, p), until all the edges are claimed. Maker wins if he claims all the edges of a graph H; Breaker wins otherwise. Recently, Müller and Stojaković determined the threshold for the graph property that Maker can win this game i...

To determine whether rod-mediated vision in goldfish is regulated by a circadian clock, absolute threshold was measured psychophysically in animals maintained in constant darkness. Responses were recorded approximately every 4 hr to a diffuse 532 nm stimulus, 5 sec in duration, with no background light present. Visual threshold tended to be lowest at the time of transition from light to dark, a...

Paraplegia may increase susceptibility to ventricular arrhythmias by altering the autonomic control of the heart. Altered cardiac autonomic control has been documented to change the expression of genes that encode cardiac Ca2+ regulatory proteins. Therefore, we tested the hypothesis that paraplegia alters cardiac electrophysiology with concomitant changes in Ca2+ regulatory proteins in a manner...