The emergence of high-throughput, massive or next-generation sequencing technologies has created a completely new foundation for molecular analyses. Various selective enrichment processes are commonly applied to facilitate detection of predefined (known) targets. Such approaches, however, inevitably introduce a bias and are prone to miss unknown targets. Here we review the application of high-t...

BACKGROUND Different high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved. METHODOLOGY/PRINCIPAL FINDINGS We describe an experimental and computational pipeline yielding millions of reads that can exceed 200 bp with quality scores approac...

UNLABELLED Novel DNA sequencing technologies with the potential for up to three orders magnitude more sequence throughput than conventional Sanger sequencing are emerging. The instrument now available from Solexa Ltd, produces millions of short DNA sequences of 25 nt each. Due to ubiquitous repeats in large genomes and the inability of short sequences to uniquely and unambiguously characterize ...

MOTIVATION During the process of high-throughput genome sequencing there are opportunities for mixups of reagents and data associated with particular projects. The sequencing templates or sequence data generated for an assembly may become contaminated with reagents or sequences from another project, resulting in poorer quality and inaccurate assemblies. RESULTS We have developed a system to a...

DNA is fragmented and the transferred strand of the transposon end oligonucleotide is covalently attached to the 5′ end of the target fragment (Fig. 1a). The size distribution of the fragments can be controlled At present, next-generation sequencing platforms use slightly different technologies for sequencing, such as pyrosequencing, sequencing by synthesis or sequencing by ligation. However, m...

Abstract Apple stem grooving virus, the causal agent of citrus tatterleaf disease, was detected for first time in Lao People’s Democratic Republic. Samples were collected from trees across southern provinces testing Australia. RNA extracted and tested using conventional real-time reverse transcription polymerase chain reactions with virus 1 59 samples tested. Viral identity confirmed by Sanger ...

Several recent studies from the field of epigenetics have combined chromatin-immunoprecipitation (ChIP) with next-generation high-throughput sequencing technologies to describe the locations of histone post-translational modifications (PTM) and DNA methylation genome-wide. While these reports begin to quench the chromatin biologists thirst for visualizing where in the genome epigenetic marks ar...

Bioinformatics) Studies on Human Chromatin using High-Throughput DNaseI Sequencing

Algorithms for Next-Generation High-Throughput Sequencing Technologies