These highly sensitive assays are based on the interaction between thyroid autoantibodies and 125I-labeled autoantigens. Serum samples are incubated with labeled thyroid peroxidase (TPO) or thyroglobulin (Tg) to allow the formation of antibody-labeled antigen complexes. The complexes are then precipitated by addition of solid-phase Protein A. In the presence of high concentrations of TPO antibo...

Thyroglobulin labeled with L-[U-‘*C]tyrosine was prepared, purified, and iodinated in order to investigate the fate of the 3-carbon fragment which remains when 2 iodotyrosine residues (18 carbon atoms) in thyroglobulin react with each other in the course of thyroid hormonogenesis to form 1 hormone residue (15 carbon atoms). Incubation of hog thyroid slices with L-[U-‘*C]tyrosine led to the form...

To the Editor: High doses of iodine-131 (131I) are commonly used in patients with differentiated thyroid cancer (DTC) after total or subtotal thyroidectomy, in order to ablate the remaining cancer or normal thyroid tissue [1]. Whole body scan performed after about 5 days can visualize possible metastatic lesions [2] and may also show the stomach, salivary glands, the breasts expressing sodium i...

A double antibody radioimmunoassay has been developed to measure thyroglobulin in rat (RTg) serum. The lowest detectable quantity measurable was 5.0 ng/ml. Specificity was documented by: (a) fall in serum RTg to undetectable levels after thyroid ablation; (b) the fact that L-thyroxine, D-thyroxine, L-triiodothyronine, D-triiodothyronine, triiodothyroacetic acid, tetraiodothyroacetic acid, triio...

Metformin has been found to reduce elevated gonadotropin levels. Hashimoto’s thyroiditis is the most common thyroid disorder in iodine-sufficient areas, and it often develops postmenopausal women. The aim of this study was investigate whether autoimmune determines impact metformin on gonadotrope secretory function. Two matched groups women were studied: 35 with euthyroid (group A) without disor...

Aim: To find out association of Serum Thyroid Stimulating Hormone (TSH) and Anti-Thyroid Antibodies (ATA) i.e., anti Tg-Ab & TPO-Ab with thyroid carcinoma in nodule patients presented Mayo Hospital Lahore. Study design: It was a comparative cross-sectional study. Methods: wasconducted Biochemistry Department Biomedical Sciences KEMU, Lahore from February 2018 to May 2019. Seventy were inclu...

Postpartum thyroiditis (PPT) is characterized by mild thyrotoxicosis occurring within one year of parturition commonly followed transient hypothyroidism. Having genetic background autoimmune thyroid disorders a risk factor for it because the immune reactivation during postpartum period trigger PPT. Pandemic COVID-19: caused SARS-CoV-2 infection global health problem, and occurrence Graves’ dise...

This retrospective study aimed to investigate whether there was a difference in the success rate of removal residual thyroid tissue patients with same cutoff serum thyroglobulin (Tg) value―measured 2 weeks after hormone withdrawal (THW)―for different radioactive iodine (RAI) activities. We identified 132 papillary cancer who were treated total thyroidectomy and RAI therapy evaluate efficacy thr...