Thyroid peroxidase (TPO) was purified from each thyroid of 10 patients (experiment 1) and 4 patients (experiment 2) with toxic diffuse goiter by a simplified method with monoclonal anti-TPO antibody-assisted immunoaffinity column chromatography. The final preparations were used to measure the heme concentration based on the cyanide difference spectrum, and to determine the Km and kcdt values fr...

We carried out immunohistochemistry and western blotting of fresh frozen sections and crude extracts from adult rat thyroids. The histochemical and immunoblotting studies were performed with P2X receptor antibodies from 2 different sources. P2X-immunopositive cells were identified by fluorescence double labelling and confocal microscopy. Results of the western blotting experiments showed double...

Knowledge concerning the structure and quality of thyroglobulin (Tg) has great significance for the better understanding of the pathogenesis of different thyroid diseases. The localization of the Tg gene and studies of its structure by molecular biological techniques make possible precise investigations of its expression. The aim of our study was to evaluate Tg content in the thyroids and Tg co...

The C cell complexes in the dog thyroid are the remnants of ultimobranchial bodies which retain fetal characteristics, and contain cyst structures showing various sizes, shapes and liminal contents. This study deals with these cysts with regard to their morphology, immunoperoxidase reactions to anti-calcitonin, anti-19S thyroglobulin and anti-C-thyroglobulin antisera, and ability to incorporate...

Peroxidase activities of autonomously functioning thyroid tumors (T) and surrounding non-tumorous tissue (N) in 5 patients were determined by employing guaiacol or iodide as the second substrates. The mean values for specific activities of T were 30 times (in iodide oxidation assay) or 4 times (in guaiacol oxidation assay) as high as those in N, being significantly higher than those of non-func...

Spontaneous thyroid tumors in salmonid fishes, which appear to originate as simple hyperplasias, according to Marine and Lenhart (10, 11) and Marine (9), ultimately become invasive and re place fibrous connective tissue, muscle, and bone. Upon treatment with iodine these tumors will regress even after they have become invasive. It is interesting to compare the tumor in fish with thyroidal growt...

background : gastric cancer is a common and lethal disease throughout the world. in iran with 7300 new cases annually, it is the first cause of cancer related death in both sexes. regarding the high incidence (10.5/100000 individuals) of gastric can­cer and priority of prevalence index in cancer management, in this study we tried to determine 1, 2-3 and 4-5 year point preva­lence of the disease...

Background Thyroid and parathyroid are two endocrine glands implicated in regulation of growth and metabolism as well as calcium homeostasis. Thyroid hypertrophy, hyperplasia and neoplasia are spontaneous findings often observed in rats in non-clinical safety studies. Such findings, however, can also be induced by investigational compounds. In order to understand the time of onset, progression ...

Broiler chickens were reared in batteries and fed diets designed to determine the effects of either ascorbic acid, aspirin (acetylsalicylic acid), lysine, or thiouracil on thyroid weight and serum thyroid hormone concentrations. Thyroxine (T4) and 3,5,5'-triiodothyronine (T3) concentrations in serum were determined by radioimmunoassay. Neither ascorbic acid nor lysine affected T3 or T4 concentr...

I t is now 16 years since Nowikoff (1908) accelerated the division rate of Paramecium by adding thyroid gland to his cultures. Later , Shumway (1914) reached the same conclusion after a series of more critical and painstaking experiments, and in the following year Budington and Harvey (1915) showed tha t thyroids from various vertebrates would produce similar results. Still later, Shumway (1917...