two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of pth-amino acids which have very close rf-values. the solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of pth-amino acids. the solvent systems provide a clear separation of all pth-amono acids except lle / leu in a rel...

Traditional medicine or folk medicine practices are based on the use of plants and plant extracts. Standardization and phytochemical investigation of the extract plays a very important role in determining the active constituents and relative purity. The objective of the present study is to investigate the phytochemical constituents, along with antioxidant evaluation and standardization of nelum...

Phenolic compounds are a group of plant secondary metabolites known to have a variety of bioactivities, including the ability to function as antioxidants. Because of the side effects of the use of synthetic substances, the search for natural and less toxic compounds has increased significantly. This study was designed to evaluate the antioxidant activity and phenol content of hexane, ethyl acet...

Planar chromatography is a very useful tool for analysis of wide range of different mixtures. Thanks to its possibility for rapid separation of large number of samples simultaneously, low solvent consumption and ability to analyse rough material allow to receive precise and reliable results in short time and low cost. Miniaturization of planar techniques brings a lot of advantages, such as shor...

Plants are still the preferred solution to solve several of main problems world is facing today, such as antibiotic resistance and free radicals. The plant chemical compounds have potential antibacterial antioxidant agents. Therefore, four plants representing Phyllantaceae family ( Glochidion varians Miq., zeylanicum (Gaertn.) A.Juss) two from Rubiaceae Uncaria lanosa var. glabrata, cordata (Lo...

This study was undertaken to evaluate the antioxidant and acetylcholinesterase inhibition properties of stems and leaves of hexane and methanolic extracts of Pluchea indica. Methanolic extract of leaves showed the highest antioxidant activity (IC50= 24.45 ± 0.34 μg/ml) and total phenolic contents (573.52 ± 6.2 mg GAE/100 g crude extract), in DPPH radical scavenging and Folin-Ciocalteu assays re...

"Bailahuén" is the common name of a medicinal shrub native to Chile where this resinous herb is widely used for its liver stimulating properties. Although the official species is Haplopappus baylahuen Remy (Asteraceae), other species of the same genus are also used in different regions as "bailahuén". A thin layer chromatography (TLC) method for rapid identification of different species and det...

Thin layer chromatography (TLC) is routinely used by synthetic organic chemists and natural products chemists working on a variety of types of molecules. Carbohydrate chemists and biochemists in the past largely relied on paper chromatography for rapid and inexpensive analysis. Carbohydrates are highly polar molecules and often require derivatization to be analyzed by TLC. Acidic carbohydrates ...

The aim of this study was to evaluate and compare the cellular antioxidant activities of Lantana montevidensis, Lippia multiflora, and Ocimum gratissimum leaves often consumed as herbal teas in a rural area of Bandundu severely affected by konzo, which is related to oxidative damage. Consequently, dietary supplements with proven antioxidant potentialities could be of real interest to promote in...