Staining of mast cells (MCs), including porcine ones, is critically dependent upon the fixation and staining technique. In the pig, mucosal and submucosal MCs do not stain or stain only faintly after formalin fixation. Some fixation methods are particularly recommended for MC staining, for example the fixation with Carnoy or lead salts. Zinc salt fixation (ZSF) has been reported to work excelle...

Although the combination of toluidine blue O (TBO) dye and laser light at a wavelength of 633 nm has a bactericidal effect, light from laboratory lasers can only be directed externally at a bacterial colony or suspension. In this study a novel delivery system guided the laser light to an 800-micrometer diameter spherical tip (an isotropic tip) from which light radiated producing a uniform spher...

BACKGROUND Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. METHODS Standard Suspensions of S. mutans were...

The cortical cytoplasm of the developing oocyte of Rana pipiens has been investigated by cytochemical means. Particular emphasis is placed on the nature and distribution of polysaccharide protein complexes. With toluidine blue O, both living and fixed mature oocytes were found to possess a distinct metachromatic region just under the developing vitelline membrane. Younger oocytes, at the previt...

A selective medium with DNase test agar and incorporating vancomycin (10 micrograms/ml), trimethoprim (8 micrograms/ml), and amphotericin B (2 micrograms/ml) supported the growth of 305 Branhamella catarrhalis isolates. A modified toluidine blue O technique was used after 48 h of incubation in CO2 to overlay suspected B. catarrhalis colonies. A metachromatic color change was observed in 15 min,...

An 18-year-old Japanese male presented with a normal-skin-colored, dome-shaped, slightly tender tumor on his left palm. Histopathologically, the tumor consisted of distinct lobules, which were separated by fibrous connective tissue. Each lobule was composed of spindle or round cells within a myxoid matrix, pathognomonic of nerve sheath myxoma. The mucinous material was positively stained by Alc...