Cancer cells display enhanced glycolysis to meet their energetic and biosynthetic demands even under normal oxygen concentrations. Recent studies have revealed that tumor suppressor p53 represses glycolysis under normoxia as a novel mechanism for tumor suppression. As a common microenvironmental stress for tumors, hypoxia drives the metabolic switch from the oxidative phosphorylation to glycoly...

We hypothesized that a better correlation between MRI and clinical measures of neurological disability using the expanded disability status scale (EDSS) in multiple sclerosis could be obtained by assessing lesion load only in and around the corticospinal tracts, since the EDSS is weighted towards motor and ambulatory deficits. Multiple sclerosis lesions in cerebral MRIs from 39 patients with re...

Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram ...

addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and atp depletion. at cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. cytochrome p-450 inhibitors inhibited peroxynitrite formation and prevented ...

Under aerobic conditions, glucose consumption by rat reticulocytes was relatively low. When cellular respiratory activity was blocked by antimycin A, both glucose uptake and lactate formation were greatly increased. Pasteur control (the ratio of the rate of glycolysis in the respiratoryinhibited state to that in the uninhibited aerobic state) was very high in these cells and varied from 9 to 12...

Stored blood components are a critical life-saving tool provided to patients by health services worldwide. Red cells may be stored for up to 42 days, allowing for efficient blood bank inventory management, but with prolonged storage comes an unwanted side-effect known as the "storage lesion", which has been implicated in poorer patient outcomes. This lesion is comprised of a number of processes...

A 36-year-old man with staphylococcal meningitis had similar symptoms and MRI characteristics as reversible splenium lesion syndrome described in the literature-hyperdensity on DWI and T2WI/FLAIR, hypodensity on ADC, iso-intensity on T1WI, and no contrast enhancement on midline splenium. The splenium lesion on MRI disappeared as the symptoms improved. The etiology of staphylococcal meningitis w...

Impaired resting metabolism in peripheral muscles potentially contributes to exercise intolerance in chronic obstructive pulmonary disease (COPD). This study investigated the cytosolic energy metabolism of the quadriceps, from glycogen degradation to lactate accumulation, in exercising patients with COPD, in comparison to healthy controls. We measured, in 12 patients with COPD and 10 control su...