The transcriptome of each individual cell contains numerous RNA species, each of which can be controlled by multiple mechanisms during their lifetime. The standard transcriptome analysis focuses on the expression levels of the genes of interest. To gain additional insights into spatiotemporal RNA distribution and the underlying trafficking processes, RNA labeling and imaging are necessary-ideal...

CRISPR-Cas9 has recently emerged as a promising system for multiplexed genome editing as well as epigenome and transcriptome perturbation. Due to its specificity, ease of use and highly modular programmable nature, it has been widely adopted for a variety of applications such as genome editing, transcriptional inhibition and activation, genetic screening, DNA localization imaging, and many more...

Citation: Pootakham W, Naktang C, Sonthirod C, Yoocha T, Sangsrakru D, Jomchai N, Putchim L and Tangphatsornruang S (2018) Development of a Novel Reference Transcriptome for Scleractinian Coral Porites lutea Using Single-Molecule Long-Read Isoform Sequencing (Iso-Seq). Front. Mar. Sci. 5:122. doi: 10.3389/fmars.2018.00122 Development of a Novel Reference Transcriptome for Scleractinian Coral Po...

CovRS Regulated Transcriptome Analysis of a Hypervirulent M23 Strain of Group A Streptococcus 1 pyogenes Provides New Insights on Virulence Determinants 2 3 Yun-Juan Bao, Zhong Liang, Jeffrey A. Mayfield, Shaun W. Lee, Victoria A. Ploplis, 4 Francis J. Castellino 5 6 W.M. Keck Center for Transgene Research and Department of Chemistry and Biochemistry, 7 Department of Biological Sciences, Univer...

Comparative Study of Transcriptome Profile and Immune-related Genes Network Associated with Intestinal Epithelial Tissue based on Microarray Data in Poultry Coccidiosis

Analyse statistique du transcriptome Programme-2-Programme 09h30-10h00 Enregistrement 10h00-10h10 Ouverture de la journée 10h10-10h30 Evidence for a dual constraint on transcriptomes : both species-specific and tissue-specific gene expression pattern are intermingled – C. Keime, L. Duret, D. Mouchiroud, O. Gandrillon 10h30-10h50 ManGO : an interactive R-based tool for two-colour microarray anal...

Canada is the world’s largest producer of canola and the trend of production is ever increasing with an annual growth rate of 9.38% according to FAOSTAT [1]. In 2017, canola acreage surpassed wheat in Saskatchewan, the highest producer of both crops in Canada. Country-wide, the total farming area of canola increased by 9.9% to 22.4 million acres while wheat area saw a slight decline to 23.3 mil...

Analyses of high-throughput transcriptome sequences of non-model organisms are based on two main approaches: de novo assembly and genome-guided assembly using mapping to assign reads prior to assembly. Given the limits of mapping reads to a reference when it is highly divergent, as is frequently the case for non-model species, we evaluate whether using blastn would outperform mapping methods fo...