Expression of drug-resistant forms of dihydrofolate reductase (DHFR) in hematopoietic cells confers substantial resistance of animals to antifolate administration. In this study, we tested whether the chemoprotection conferred by expression of the tyrosine-22 variant DHFR could be used for more effective therapy of the 32Dp210 murine model of chronic myeloid leukemia (CML). Administration of th...

Expression of drug-resistant forms of dihydrofolate reductase (DHFR) in hematopoietic cells confers substantial resistance of animals to antifolate administration. In this study, we tested whether the chemoprotection conferred by expression of the tyrosine-22 variant DHFR could be used for more effective therapy of the 32Dp210 murine model of chronic myeloid leukemia (CML). Administration of th...

Adalimumab is the only FDA- and EMA-approved treatment for moderate to severe hidradenitis suppurativa (HS), where etanercept certolizumab-pegol have been shown be inefficacious, suggesting that mechanism of action (MOA) adalimumab distinct in HS may contribute improved wound healing. We demonstrated adalimumab, but neither nor certolizumab-pegol, induces a healing profile vitro by regulating m...

(co) variance components and genetic parameters for growth traits in arman sheep were estimated, using data collected during an 11-year period (1999-2010), by applying the restricted maximum likelihood (reml) procedure under univariate and bivariate animal models. the studied traits were body weight of lamb at birth (bw), body weight at 3 months of age as weaning weight (ww), body weight at 6 m...

Transgenic animal technology, which allows the germline insertion of exogenous genes or the alteration or disruption of endogenous genes, has emerged as a powerful tool for the in vivo analysis of gene function. Since the primary strategy of transgenic techniques is to examine the biological results of lifetime overproduction or underproduction of particular gene products, perhaps no field is b...

Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition...

15 16 17 The birth of the first transgenic primate to have inherited a transgene from its parents 18 opens the possibility to set up transgenic marmoset colonies, as these monkeys are small 19 and relatively easy to keep and breed in research facilities. The prospect of transgenic 20 marmoset models of human disease, readily available in the way that transgenic 21 laboratory mice are currently,...

The rabbit has long been used as a laboratory animal model for developing reproductive and stem cell-related technologies, as well as for studying human disease. The Oct4 transcription factor plays a crucial role in the maintenance and regulation of pluripotency in embryos and stem cells. We constructed a reporter plasmid containing the gene encoding the enhanced green fluorescent protein (EGFP...

While the chimpanzee remains the only animal that closely models human hepatitis C virus (HCV) infection, transgenic and immunodeficient mice in which human liver can be engrafted serve as a partial solution to the need for a small animal model for HCV infection. The established system that was based on mice carrying a transgene for urokinase-type plasminogen activator (uPA) gene under the cont...

Background We previously showed the properties of interleukin-1b antagonist Anakinra on unmyelinated nerve fibers protection in a transgenic mouse model for human V30M transthyretin. In these studies, Anakinra decreased, among other markers, nerve levels of IL-1b, NFB and activated-caspase 3, associated with a decrease in TTRnon fibrillar deposition. In the present work, we compared the efficac...