In this study, high performance liquid chromatography (HPLC) was used to analyze the protodioscin content in Tribulus terrestris samples. They were separated by C18 column, gradient with 0.1 % phosphoric acid solution and acetonitrile as mobile phase flow rate of 0.5 mL/min, column temperature 45 °C, detection wavelength 203 nm. The results showed that has a good linear correlation (r>0.999)...
