igenesis, abnormally hypermethylated or hypomethylated sequences occurring in the tumor genome need to be identified. In this study, we have developed a PCR-based method, called MSRF,3 to identify abnormally methylated CpG sites in breast carci nomas. Initially, genomic DNA was digested with a 4-base restriction endonuclease known to cut bulk DNA into small fragments but rarely to cut in the CG...

New approaches have recently been developed for directly identifying genes without knowledge about their function and/or position in a genome (1–3). Among these, representational difference analysis (RDA) (2) is widely used in the detection of deleted DNA in cultured cancer cells (4). However, the procedure is complicated and time-consuming. First, preparation of the tester and the driver invol...

HeLa cells incubated in serum-free medium accumulated 59Fe ("non-transferrin iron") when incubated with either 59Fe-citrate, 59Fe-nitrilotriacetate, or 59Fe dissolved in Tricine ascorbate. Accumulation of iron was time-, concentration-, and Ca2+-dependent and was saturable. Uptake of non-transferrin (non-Tf) iron was transferrin-independent because of the fact that uptake occurred at pH 5.5, a ...

UNLABELLED With only 19 amino acids, duramycin is the smallest known polypeptide that has a defined 3-dimensional binding structure. Duramycin binds phosphatidylethanolamine (PtdE) at a 1:1 ratio with high affinity and exclusive specificity. As an abundant binding target, PtdE is a major phospholipid and accounts for about 20% of the phospholipid content in mammalian cellular membranes. PtdE is...

UNLABELLED We developed a novel integrated quad-modality system that included 3 molecular imaging methods (PET, SPECT, and fluorescence molecular imaging [FMI]) and 1 anatomic imaging modality (CT). This system could study various biologic processes in the same animal using multiple molecular tracers. In addition to the technology development, we also discussed the optimization strategy of the ...

Components of the proteinaceous cement secreted by barnacles have yet to be studied because of their insolubility. We solubilized and characterized the proteins of secondary cement, which is produced when the barnacle is detached from the substratum, in Megabalanus rosa. The cement was fractionated, according to its solubility in aqueous formic acid, into a soluble fraction, SF1 (2 1%); a fract...

1 The cell wall fraction of the gram-positive, non-toxic Corynebacterium diphtheriae strain 2 C8r(-) Tox (ATCC 11913) contained a channel-forming protein as judged from reconstitution 3 experiments with artificial lipid bilayer experiments. The channel-forming protein was present 4 in detergent treated cell walls and in extracts of whole cells using organic solvents. The 5 protein had an appare...

The dimeric enzyme creatine kinase (EC 2.7.3.2) from rabbit muscle has a reactive thiol group on each subunit, modification of which by iodoacetate or iodoacetamide leads to inactivation of the enzyme (Watts, 1973). It had been previously shown (Price & Hunter, 1976) that when the enzyme is treated with iodoacetate or some other reagents in the presence of the 'transition-state analogue' comple...

Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, that besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which are well tolerated, most of the antifungal drugs used for candidiasis treatment can cause side effects and lead to th...

This work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (LAB) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. The two bacteriocinogenic isolates were identified by Polymerase Chain Reaction (PCR) using genus- and species-specific primers, and confirmed by 16S rDNA sequencing, as Entero...