of trypsin without EDTA. A monolayer of radioactively labelled BHK cells was incubated at 37°C for2min in 0.25 % trypsin; one half of the cells in suspension were harvested, washed and assayed for adhesion, and the other half of the cells were incubated for a further 15min in 0.25% trypsin before harvesting, washing and assaying for adhesion. Cells incubated in trypsin for only 2min did not sho...

Peptide mapping of antibodies is an essential method to monitor peptide modifications in antibody lots that could affect the safety and efficacy product. Conventional protocols rely on protein digestion using proteases, such as trypsin, before with mass spectrometry (MS). However, trypsin may cause incomplete peptides, especially those include highly hydrophobic peptides. Here, we show how peps...

Insulin did not stimulate glucose metabolism or inhibit lipolysis in brown fat cells isolated by digestion of rat interscapular brown adipose tissue with collagenase plus 1 mg of trypsin per ml. Trypsin-treated cells responded normally to catecholamines and to the antilipolytic agent, prostaglandin El. The stimulation of respiration by lipolytic agents and free fatty acids was not affected by t...

Understanding molecular recognition on a structural basis is an objective with broad academic and applied significance. In the complexes of serine proteases and their proteinaceous inhibitors, recognition is governed mainly by residue P1 in accord with primary serine protease specificity. The bifunctional soybean Bowman-Birk inhibitor (sBBI) should, therefore, interact at LysI16 (subdomain 1) w...

Genetic elimination of kunitz trypsin inhibitor in soybean seed would obviate the need for boiling required to inactivate the antinutritional factor and therefore economize the soy processing. PI542044, the source of null variant of kunitz trypsin inhibitor gene is being used in the development of kunitz trypsin inhibitor free soybean genotypes in India. Gene specific marker can expedite the ge...

BACKGROUND AND AIMS Endoprotease activation is a key step in acute pancreatitis and early inhibition of these enzymes may protect from organ damage. In vivo models commonly used to evaluate protease inhibitors require animal sacrifice and therefore limit the assessment of dynamic processes. Here, we established a non-invasive fluorescence imaging-based biomarker assay to assess real-time protea...

The proteins and trypsin inhibitors were isolated from the seeds of different varieties/accessions of an underutilized legume, Mucuna. The crude protein content of all the germplasms of Mucuna is varied from 15% 26%, showed little variation and contain higher crude protein when compared with other Mucuna species reported earlier and the pulse crops commonly consumed in India. The seeds of all t...

Linitis plastica, or Borrmann 4 gastric cancer, shows very poor prognosis, and the reason has not been understood. In the present study, we examined serum levels of trypsin(ogen) in 44 gastric cancer patients, including 17 early gastric cancer, 18 non-Borrmann 4 advanced gastric cancer, and 9 Borrmann 4 gastric cancer, by using the RIA gnost Trypsin kit (Hoechst Japan, Tokyo, Japan), which was ...

To further characterize the nature of proteolytic processing of the astrovirus capsid, we infected Caco-2 cells with a high multiplicity of astrovirus without trypsin in the presence of 5 to 10% fetal calf serum. These infections were characterized by pulse-chase labeling with [35S]Smethionine, electron microscopy, gel electrophoresis of purified viral particles, and analysis of infectivity of ...

PURPOSE To improve the 30-year-old "trypsin digestion" procedure for isolation of the complete retinal vasculature, which, in its time, was a revolutionary advance that allowed important discoveries about diabetic retinopathy; to provide a method that will yield more consistent results when applied to retinas representing a wide range of ages, species, and severity of vascular disease, such as ...