Tremendous progress has been made in understanding the functions of γ-tubulin and, in particular, its role in microtubule nucleation since the publication of its discovery in 1989. The structure of γ-tubulin has been determined, and the components of γ-tubulin complexes have been identified. Significant progress in understanding the structure of the γ-tubulin ring complex and its components has...

Bovine rod outer segment (ROS) preparations contain a major 58 kDa protein doublet that was identified by immunoblot as tubulin. Quantification by gel densitometry showed that the total amount of tubulin was 5- to 10-fold higher than that attributable to the rod axoneme, suggesting additional role(s) for tubulin in photoreceptor cells. Approximately 20% of this nonaxonemal tubulin (15% of total...

The dynamics of microtubules (MT's) growing from a nucleation center is simulated with a kinetic Monte Carlo model that includes tubulin diffusion. In the limit of fast diffusion (homogeneous tubulin concentration), MT growth is synchronous and bounded. The microtubules form an aster with a monotonously decreasing long-time distribution of lengths. Slow tubulin diffusion leads to rapid dephasin...

during spring and summer of 2010 and 2012, several surveys were conducted in different vineyards in kohgiluyeh & boyer-ahmad (southwestern iran) and kerman (southeastern iran) provinces, with the aim of isolating and identifying fungi associated with grapevine decline diseases. the samples collected comprised diseased grapevines showing yellowing, defoliation, reduced growth, wilting, intervein...

surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. in this study beta tubulin gene (bt2), was used as the first time in a pcr-rflp format to clarify the distribution of dermatophytosis agents in some parts of iran.a total of 603 clinical isolates was obtained from 500 patients in tehran, isfahan, mazanda...

b ackground: due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new pcr-rflp technique, based on a modified forward primer (ut- hc167 mf-primer), to identify simultaneously the snps at codons 167 and 200 of isotype 1 β-tubu- lin gene of haemonchus contortus . m ethods: there already are several safe and easy methods for identificati...

Seven monoclonal antibodies raised against tubulin from the axonemes of sea urchin sperm flagella recognize an acetylated form of alpha-tubulin present in the axoneme of a variety of organisms. The antigen was not detected among soluble, cytoplasmic alpha-tubulin isoforms from a variety of cells. The specificity of the antibodies was determined by in vitro acetylation of sea urchin and Chlamydo...

Tubulin, the dimeric subunit of microtubules, is a major cell protein that is centrally involved in cell division. Tubulin is subject to specific enzymatic posttranslational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha-subunit. Tubulin is normally extensively tyrosinated in cycling cells. However, we have previously shown that detyrosinated tubu...

Downlo helial-to-mesenchymal transition (EMT) is associated with increased breast tumor metastasis; however, ecific mechanisms by which EMT promotes metastasis remain somewhat unclear. Despite the imporof cytoskeletal dynamics during both EMT and metastasis, very few current studies examine the cyton of detached and circulating tumor cells. Specific posttranslational α-tubulin modifications are...

Porcine brain tubulin labeled with fluorescein isothiocyanate (FITC) was able to polymerize by itself and co-polymerize with tubulin purified from starfish sperm flagella. When we injected the FITC-labeled tubulin into unfertilized eggs of the sand dollar, Clypeaster japonicus, and the eggs were then fertilized, the labeled tubulin was incorporated into the sperm aster. When injected into ferti...