A cDNA library of Limax flavus was constructed and screened for sialic-acid-specific lectins. Complementary DNA clones were categorized into seven groups corresponding to closely related but different sequences. Group 1 clones contained an ORF encoding 199 amino acids including a sequence identical to the partial amino acid sequence obtained from the lectin protein. Within its 1074-bp 3' untran...

Human prostasin is a membrane-anchored serine peptidase hypothesized to regulate lung epithelial sodium transport. It belongs to a unique family of genes on chromosome 16p11.2/13.3. Here we describe genomic cloning, promoter analysis, and expression of prostasin's mouse ortholog. The 4.3-kb mouse prostasin gene (prss8) has a six-exon organization identical to human prostasin. Prss8 spans two si...

Retrotransposons contribute significantly to the evolution of eukaryotic genomes. They replicate by producing DNA copies of their own RNA, which are integrated at new locations in the host cell genome. In the gene-dense genome of the social amoeba Dictyostelium discoideum, retrotransposon TRE5-A avoids insertional mutagenesis by targeting the transcription factor (TF) IIIC/IIIB complex and inte...

The chromosomal gene for human interleukin 1 alpha (IL-1 alpha) was isolated from a human genomic DNA library by using as a probe cloned human IL-1 alpha cDNA. Complete nucleotide sequence of about 12 kilobase pairs (kbp) long was determined and the structure and organization of this gene were elucidated. This gene contains seven exons and six introns. The first exon encodes the 5'-untranslated...

In a patient with partial androgen insensitivity syndrome (AIS), we identified a single inherited presumably silent nucleotide variation (AGC -> AGT) in exon 8 (codon 888) of the AR gene. However, in the patient's genital skin fibroblasts, a considerably shortened transcript of 5.5 kb (normal: 10.5 kb) was detected, which misses a part of exon 8 and a prominent portion of the 3'-untranslated re...

The techniques of indirect immunofluorescence (IF), immuno-peroxidase (IP) staining and the one-step reverse transcriptase polymerase chain reaction (RT-PCR) were compared for detection of 102 isolates of bovine viral diarrhoea virus (BVDV) in infected cell cultures. The BVDV was obtained from bovine clinical specimens, including sera, buffy coats and tissues, submitted from farms located in th...

conclusions sequence and structural diversity of the 3'utr variable region and poly u/uc region found in the local isolates indicate specificity in the regulating elements of 3'utr that might be associated with differential replication efficacy of the hcv pakistani isolates. the study necessitates functional characterization of these regulating elements to elucidate variable viral eff...

Several cDNAs encoding H-protein, a constituent of the glycine cleavage system, were cloned from chicken liver cDNA libraries with an antibody raised against rat H-protein or with a nick-translated cDNA of an immunoreactive clone. The structure of the H-protein cDNA consisting of 910 base pairs was determined using clones with an apparent overlap in the nucleotide sequence. The cDNA encodes the...

Abbreviations: DMS: Dimethyl Sulphate; ds: Double-Strand; HCV: Hepatitis C Virus; NMIA: N-Methylisatoic Anhydride; NS: Nonstructural; ORF: Open Reading Frame; RT-qPCR: Reverse Transcription-Quantitative Polymerase Chain Reaction; SD: Standard Deviation; SEM: Standard Error of the Mean; SL: Stem-Loop; ss: SingleStrand; SHAPE: Selective 2'-hydroxyl Acylation Analyzed by Primer Extension; UTR: Unt...

Feline immunodeficiency virus (FIV) has worldwide distribution; nevertheless, only a few FIV genomes from domestic cats are available. This is the first report of a nearly complete genome of FIV from a Brazilian cat (8,967 nucleotides [nt]), including the entire coding region and the 3' untranslated region.