because of the huge amounts of proteomic data and demand for new methods of laboratory analysis results, proteins collective analysis, in addition to taking less time, biostatistician assist at identification of new patterns in the data set. in this study, rat hippocampus proteome in normal and alzheimer's disease (ad) were analyzed by using proteomic techniques and bioinformatics’ analysis. pr...

OBJECTIVE Proteinuria is the hallmark of diabetic nephropathy; yet, glomerular histology does not fully explain mechanisms contributing to proteinuria. Our objective was to identify proteins in the urine of individuals with type 1 diabetes and microalbuminuria that might implicate a mechanistic pathway operative in proteinuria. RESEARCH DESIGN AND METHODS Using a GeLC/MS platform proteomics a...

The spread of multidrug-resistant microorganisms globally has created an urgent need for novel therapeutic strategies to combat urinary tract infections (UTIs). Immunomodulatory therapy may provide benefit, as treatment of mice with dexamethasone during acute UTI improved outcome by reducing the development of chronic cystitis, which predisposes to recurrent infection. Here we discovered solubl...

Urinary tract obstruction is a frequent cause of renal impairment. The physiopathology of obstructive nephropathy has long been viewed as a mere mechanical problem. However, recent advances in cell and systems biology have disclosed a complex physiopathology involving a high number of molecular mediators of injury that lead to cellular processes of apoptotic cell death, cell injury leading to i...

The analysis of historical materials is an essential component cultural heritage conservation. Protein was one the most important and pervasive organic substances in ancient human societies. Through qualitative quantitative examination protein-based materials, it possible to clarify their source functions heritages, investigate manufacturing technology heritage, identify deterioration mechanism...

  The Nobel Prize in Chemistry for 2002 was shared by John B. Fenn and Koichi Tanaka "for their development of soft desorption methods for mass spectrometric analyses of biological macromolecules". Indeed, electrospray ionization and soft laser desorption ionization have proved to be of great value in "omics", such as metabolomics, transcriptomics and proteomics in providing a systematic and qu...

It has been estimated that globally there is a death attributable to prostate cancer every four minutes. As life expectancy in all world regions increases, so too incidence of this disease of the ageing male will increase. For many men diagnosis occurs after presentation with symptoms of altered urinary dynamics. Unfortunately, these changes, whilst also associated with benign disease, are evid...

Animal production and health are of significant economic importance, particularly regarding the world food supply. veterinary sciences have evolved immensely in past six decades, genetics, nutrition, housing, management health. To address major challenges such as those posed by climate change or metabolic disorders, it is utmost importance to use state-of-the-art research tools. Proteomics othe...

background: due to difficulty of obtaining a 24h urine (especially in children), a random urine calcium sample is recommended to detect of hypercalciuria. however, recent studies have shown that the urinary calcium/creatinine ratio varies with age and geographic areas. so, the aim of this study was determining the normal value of urinary calcium to creatinine ratio in healthy adolescent’s child...

Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist. The events that have led to the birth of proteomics ...