(Figure 74) the same thrombin concentration (0.05 U/mL, 5 minutes) increased cGMP content in WT mouse platelets only 3-fold (from 300 to 900 fmol/108 platelets), whereas in Zhang et al,1 it increased the cGMP content 13-fold (from 100 to 1300 pmol/108 platelets), which is comparable with the cGMP increase induced by 10 M sodium nitroprusside (SNP; Figure 1D1). Second, if these data are correct,...

(Figure 74) the same thrombin concentration (0.05 U/mL, 5 minutes) increased cGMP content in WT mouse platelets only 3-fold (from 300 to 900 fmol/108 platelets), whereas in Zhang et al,1 it increased the cGMP content 13-fold (from 100 to 1300 pmol/108 platelets), which is comparable with the cGMP increase induced by 10 M sodium nitroprusside (SNP; Figure 1D1). Second, if these data are correct,...

(Figure 74) the same thrombin concentration (0.05 U/mL, 5 minutes) increased cGMP content in WT mouse platelets only 3-fold (from 300 to 900 fmol/108 platelets), whereas in Zhang et al,1 it increased the cGMP content 13-fold (from 100 to 1300 pmol/108 platelets), which is comparable with the cGMP increase induced by 10 M sodium nitroprusside (SNP; Figure 1D1). Second, if these data are correct,...

M igrating cells move forward by harnessing the force of actin polymerization to form a protru-sive lamellipodium at the leading edge. A protein called Lamellipodin (Lpd) promotes lamellipodium formation, but how it does so is unclear. Law et al. reveal that Lpd generates membrane protrusions in association with a key regulator of actin branching called the Scar/WAVE complex and that this inter...

The Ena/VASP (enabled/vasodilator stimulated phosphoprotein) family includes the binding actin proteins such as mammalian Ena (Mena), VASP, and Ena-VASP-like. It is known that the perturbation of actin cycle could determine alteration in the mobility of cells and in consequence of organogenesis. Few recent studies have revealed that Mena protein could play a role in breast or pancreatic carcino...

The Ena-VASP family of proteins act as molecular adaptors linking the cytoskeletal system to signal transduction pathways. Their N-terminal EVH1 domains use groups of exposed aromatic residues to specifically recognize 'FPPPP' motifs found in the mammalian zyxin and vinculin proteins, and ActA protein of the intracellular bacterium Listeria monocytogenes. Here, evidence is provided that the aff...

Integrins are fundamental to the control of protrusion and motility in adherent cells. However, the mechanisms by which specific members of this receptor family cooperate in signaling to cytoskeletal and adhesion dynamics are poorly understood. Here, we show that the loss of beta3 integrin in fibroblasts results in enhanced focal adhesion turnover and migration speed but impaired directional mo...

BACKGROUND/AIMS Vascular relaxation caused by Triiodothyronine (T3) involves direct activation of endothelial cells (EC) and vascular smooth muscle cells (VSMC). Activation of protein kinase G (PKG) has risen as a novel contributor to the vasorelaxation mechanism triggered by numerous stimuli. We hypothesize that T3-induced vasorelaxation involves PKG/vasodilator-stimulated phosphoprotein (VASP...