The goal of this study was to compare expression of chondrocyte-associated antigen (CAA) and cartilage matrix molecules in 2-D (monolayer) and 3-D (Matrigel) culture. Chondrocytes isolated from the cartilage of 3-day-old rats were expanded in monolayer culture for 28 days. CAA expression gradually decreased and was not detected beyond the 96th hour of monolayer culture. Collagen type II and agg...

BACKGROUND Airway remodeling in COPD includes reorganization of the extracellular matrix. Proteoglycans play a crucial role in this process as regulators of the integrity of the extracellular matrix. Altered proteoglycan immunostaining has been demonstrated in COPD lungs and this has been suggested to contribute to the pathogenesis. The major cell type responsible for production and maintenance...

The hair follicle (HF) undergoes cycles of growth (anagen), regression (catagen) and rest (telogen) phases. Both chronic reactive loss are linked to a dysfunction the HF cycle leading premature loss. Therefore, use specific active ingredients targeting could improve aim this study was evaluate efficacy 3 on anchorage in dermal papilla cells isolated from human (DPC): new patented extract Silybu...

The digital laminae is a two layer tissue that attaches the distal phalanx to the inner hoof wall, thus suspending the horse's axial skeleton in the hoof capsule. This tissue fails at the epidermal:dermal junction in laminitic horses, causing crippling disease. Basal epithelial cells line the laminar epidermal:dermal junction, undergo physiological change in laminitic horses, and lose versican ...

OBJECTIVE Whether distal inflammation in asthmatics also leads to structural changes in the alveolar parenchyma remains poorly examined, especially in patients with uncontrolled asthma. We hypothesized that patients who do not respond to conventional inhaled corticosteroid therapy have a distinct tissue composition, not only in central, but also in distal lung. METHODS Bronchial and transbron...

ADAMTS20 (Adisintegrin-like and metalloprotease domain with thrombospondin type-1 motifs) is a member of a family of secreted metalloproteases that can process a variety of extracellular matrix (ECM) components and secreted molecules. Adamts20 mutations in belted (bt) mice cause white spotting of the dorsal and ventral torso, indicative of defective neural crest (NC)-derived melanoblast develop...