Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. V. parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked, or mishandled marine products. In rare cases, V. parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-exi...

In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilitie...

The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, V...

A chemically defined medium capable of supporting the production of heat-stable and heat-labile hemolysins by Vibrio parahaemolyticus is described. The indispensability of serine and glutamic acid for hemolysin production is also demonstrated.

Screening with methyl-alpha-d-glucoside was an efficient procedure for enrichment of mutants lacking the glucose transport system and of the pleiotropic mutants lacking the phosphoenolpyruvate-dependent phosphotransferase system in Vibrio parahaemolyticus.

  This study was carried out to investigate the occurrence of potentially pathogenic species of vibrio in sea water and estuarine environments of the Caspian Sea in the Golestan province of Iran. A total of 127 water samples from coastal waters as well as from river and estuaries were collected and analyzed by culture, during April and September 2001. Following prompt centrifugation, the resusp...

Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease caused by pirAB toxins encoded by a plasmid found in Vibrio parahaemolyticus. The pirAB toxins are the homologs of the Photorhabdus insect-related (Pir) toxins. Here, we report the complete sequences of the AHPND-causing plasmid isolated from V. owensii, as well as those of its 11 siblings (pVH family). In additi...

In recent decades, the identification of small non-coding RNAs in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. In the family Vibrionaceae, which includes several human and animal pathogens, small non-coding RNAs (sRNAs) are closely related to important processes including metabolism,...

An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of ...

The phenotypic characterization and antibiotic susceptibility patterns of 72 environmental isolates of Vibrio spp. collected from six aquaculture sites along the west coast of Sabah, Borneo, Malaysia was investigated. Up to 21 conventional biochemical tests were carried out and the sensitivity of the isolates against 16 antibiotics were tested using the disk diffusion method. In the present stu...